Development and validation of analytical method for the estimation of lamivudine in rabbit plasma

来源 :Journal of Pharmaceutical Analysis | 被引量 : 0次 | 上传用户:zh0518
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Lamivudine has been widely used in the treatment of HIV disease.A reliable,sensitive reversed phase high performance liquid chromatography(RP-HPLC)method was developed and validated for lamivudine in rabbit plasma.The method was developed on Hypersil BDS C-18 column(250 mm 4.6 mm,5 mm)using a mobile phase of 0.25%Triethylamine buffer(pH 3.0): acetonitrile(70:30,v/v).The efficient was monitored by UV detector at 256 nm.The total run time was 15 min with a flow rate of 1.0 mL/min.Calibration curve was linear over the concentration range of 25-2000 ng/mL.The retention times of lamivudine and internal standard(Nelfinavir)were 8.78 min and 10.86 min,respectively.The developed RP-HPLC method can be successfully applied for the quantitative pharmacokinetic parameters determination of lamivudine in rabbit model. Lamivudine has been widely used in the treatment of HIV disease. A reliable, selected reversed phase high performance liquid chromatography (RP-HPLC) method was developed and validated for lamivudine in rabbit plasma. The method was developed on Hypersil BDS C-18 column 250 mm 4.6 mm, 5 mm) using a mobile phase of 0.25% Triethylamine buffer (pH 3.0): acetonitrile (70:30, v / v). The efficient was monitored by UV detector at 256 nm. min with a flow rate of 1.0 mL / min. Calibration curve was linear over the concentration range of 25-2000 ng / mL. The retention times of lamivudine and internal standard (Nelfinavir) were 8.78 min and 10.86 min, respectively. -HPLC method can be successfully applied for the quantitative pharmacokinetic parameters determination of lamivudine in rabbit model.
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