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目的：研究槲皮素对ＴＮＦα诱导的内皮细胞与中性粒细胞粘附的抑制作用及作用机制。方法：用髓过氧化酶法测定中性粒细胞与内皮细胞的粘附，ＥＬＩＳＡ法测定内皮细胞粘附分子的表达。结果：ＴＮＦα通过增加／诱导内皮细胞ＩＣＡＭ１，ＶＣＡＭ１及Ｅｓｅｌｅｃｔｉｎ的表达而剂量、时间依赖性地增加内皮细胞与中性粒细胞的粘附。槲皮素可剂量依赖性地抑制上述粘附，其作用机制为抑制ＴＮＦα诱导内皮细胞上述３种粘附分子的表达。结论：槲皮素通过抑制ＩＣＡＭ１，ＶＣＡＭ１及Ｅｓｅｌｅｃｔｉｎ的表达而降低ＴＮＦα诱导的内皮细胞与中性粒细胞的粘附。
Objective: To study the inhibitory effect of quercetin on TNFα-induced endothelial cell and neutrophil adhesion and its mechanism. Methods: The myeloperoxidase method was used to determine the adhesion of neutrophils to endothelial cells. The expression of endothelial cell adhesion molecules was measured by ELISA. RESULTS: TNFα increased the adhesion of endothelial cells to neutrophils in a time-and dose-dependent manner by increasing / inducing the expression of ICAM-1, VCAM-1 and E-selectin in endothelial cells. Quercetin inhibits the above adhesion in a dose-dependent manner, and its mechanism of action is to inhibit the TNFα-induced expression of the above three types of adhesion molecules in endothelial cells. Conclusion: Quercetin can reduce the TNFα-induced adhesion of endothelial cells to neutrophils by inhibiting the expression of ICAM-1, VCAM-1 and E-selectin.