论文部分内容阅读
目的:研究药用植物朱砂根组培快繁技术,为其产业化生产提供科学依据。方法:考察不同基本培养基、植物激素、添加物等对腋芽诱导、增殖及植株再生的影响。结果:以朱砂根带芽茎段为外植体,初代培养腋芽诱导的最佳培养基为MS+6-BA 0.5 mg.L-1+NAA 0.1 mg.L-1。腋芽增殖的最佳培养基配方为MS+6-BA 2.0 mg.L-1+NAA 0.1 mg.L-1+KT 0.5 mg.L-1。生根培养基为1/2MS+IBA 0.2 mg.L-1;添加0.2%的活性炭可明显促进根的生长,提高生根率、生根数。最有利于朱砂根无菌苗移栽存活的基质类型为河沙-珍珠岩-蛭石(1∶1∶1),或蛭石-珍珠岩(1∶1),成活率在80%以上。结论:通过腋芽增殖快繁育苗技术可获得完整植株,达到快速繁殖的目的。
Objective: To study the technology of tissue culture and rapid propagation of medicinal plant cinnabar root to provide a scientific basis for its industrialization. Methods: The effects of different basic media, plant hormones and additives on the induction, proliferation and plant regeneration of axillary buds were investigated. Results: The best culture medium for axillary bud induction was MS + 6-BA 0.5 mg.L-1 + NAA 0.1 mg.L-1 with roots and shoots of explants as explants. The best culture medium for axillary bud proliferation was MS + 6-BA 2.0 mg.L-1 + NAA 0.1 mg.L-1 + KT 0.5 mg.L-1. The rooting medium was 1 / 2MS + IBA 0.2 mg.L-1. Adding 0.2% activated carbon could obviously promote root growth and rooting rate and rooting number. The most favorable substrate type for survival of transplanting aseptic seedlings of cinnabar root was river sand - perlite - vermiculite (1: 1: 1) or vermiculite - perlite (1: 1) with the survival rate above 80%. Conclusion: The whole plant can be obtained through axillary bud multiplication and rapid propagation technology to achieve rapid propagation.