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目的分析近年来宁波市乙型流行性感冒(流感),两大谱系毒株流行情况和病毒血凝素基因特性。方法用犬肾传代细胞(MDCK)分离流感病毒,对2003-2009年分离的乙型流感病毒按不同的谱系选择每年的代表株,经聚合酶链反应扩增目的基因后进行乙型流感病毒的血凝素基因HA1区域(HA1)核苷酸序列测定。结果乙型流感两大谱系的病毒在宁波市几乎每年均有出现,相互交替成为优势株。其中Yamagata系在2004及2007年相对占优势,而Victoria系在2006年3月及2009年6月2次出现了流行高峰。HA1序列测定结果Yamagata系毒株在2003、2007年HA1区出现2次出现明显的氨基酸替换,Victoria系在2005年底及2009年初发生2次明显氨基酸替换。结论2003-2009年乙型流感的两大谱系病毒的HA1序列均存在较大差异,本市2004及2007年Yamagata系2次在乙型流感中相对占优势和2006及2009年Victoria系出现的2次乙型流感流行高峰均与病毒HA1区的氨基酸替换有关。
Objective To analyze the prevalence of influenza B (influenza) and the genealogy of two hemagglutinin strains in Ningbo City in recent years. Methods The influenza virus was isolated from canine kidney passage cells (MDCK). The representative strains of influenza B virus isolated from 2003 to 2009 were selected according to different lineages. The target genes were amplified by polymerase chain reaction (PCR) Hemagglutinin gene HA1 region (HA1) nucleotide sequence determination. Results The viruses of the two major lineages of Influenza B emerged almost every year in Ningbo City, alternating with each other becoming the dominant strain. The Yamagata line was relatively dominant in 2004 and 2007, while the Victoria line peaked twice in March 2006 and June 2009. Results of HA1 sequence analysis Two obvious amino acid substitutions occurred in the HA1 region of Yamagata strain in 2003 and 2007, and two obvious amino acid substitutions occurred in Victoria strain in late 2005 and early 2009. Conclusions The HA1 sequences of the two major lineage viruses of influenza B between 2003 and 2009 are quite different. The Yamagata lines in 2004 and 2007 in Yunnan Province were relatively dominant in influenza B twice and in Victoria line in 2006 and 2009 respectively The prevalence of subtype B influenza was related to the amino acid substitution in the HA1 region of the virus.