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【目的】探讨黄芩苷对高脂血症模型代谢性炎症及肠道菌群的调节作用。【方法】选取32只C57BL/6J雄性小鼠随机平均分为正常组、模型组、黄芩苷高剂量组(剂量为50 mg·kg-1·d-1)、黄芩苷低剂量组(剂量为25 mg·kg-1·d-1)。高脂饲料喂养3周后,2个黄芩苷用药组分别给予不同浓度黄芩苷灌胃5周。采用酶联免疫吸附法检测各组小鼠血清血脂水平以及肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)及内毒素水平,采用Miseq平台对小鼠肠道粪便微生物进行基因测序。【结果】与正常组比较,模型组总胆固醇及低密度脂蛋白水平显著升高(P<0.01),高密度脂蛋白水平显著降低(P<0.01),血清TNF-α、IL-6和内毒素水平显著升高(P<0.01);黄芩苷高剂量组的TNF-α、IL-6、内毒素水平均较模型组显著降低(P<0.05或P<0.01),黄芩苷低剂量组内毒素水平亦较模型组显著降低(P<0.01)。测序生物信息学分析发现,在门的水平,模型组脱铁杆菌门和变形杆菌门数目较正常组显著增多,疣微菌门数目较正常组显著减少。在属的水平,模型组Christensenella、uncultured_Peptococcaceae、脱硫弧菌属数目较正常组显著增多,而粪球菌属、Akkermansia及uncultured_Lachnospiraceae数目较正常组显著减少;黄芩苷高、低剂量组脱硫弧菌属丰度均较模型组降低。各组革兰氏阴性菌与革兰氏阳性菌数目之比,模型组较正常组显著升高(P<0.05),黄芩苷低剂量组较模型组显著降低(P<0.05)。【结论】黄芩苷能治疗高脂饮食引起的代谢性炎症,这可能与黄芩苷能调节肠道菌群的结构有关。
【Objective】 To investigate the regulatory effect of baicalin on metabolic inflammation and intestinal microflora in hyperlipidemia model. 【Methods】 Thirty-two C57BL / 6J male mice were randomly divided into normal group, model group, baicalin high-dose group (50 mg · kg-1 · d-1), low dose baicalin group 25 mg · kg -1 · d -1). Three weeks after the high-fat diet was fed, the two baicalin groups were given different concentrations of baicalin respectively for 5 weeks. The levels of serum lipids, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and endotoxin in mice were detected by enzyme-linked immunosorbent assay (ELISA) Microorganism gene sequencing. 【Results】 Compared with the normal group, the level of total cholesterol and low density lipoprotein in the model group was significantly increased (P <0.01), the level of high density lipoprotein was significantly decreased (P <0.01), the level of serum TNF-α, IL- The levels of TNF-α, IL-6 and endotoxin in the baicalin high-dose group were significantly lower than those in the model group (P <0.05 or P <0.01) Toxins were also significantly lower than the model group (P <0.01). Sequencing bioinformatics analysis showed that at the level of the door, the number of the number of the de-esobacterium and the proteobacteria in the model group was significantly higher than that in the normal group, and the number of the group of the number of the wartime-type bacteria was significantly decreased compared with the normal group. At the genus level, the number of the group of Christensenella, uncultured_Peptococcaceae and the number of desulfovibrio in the model group increased significantly compared with the normal group, while the number of the genus Faecium, Akkermansia and uncultured_Lachnospiraceae was significantly decreased compared with the normal group; Lower than the model group. The ratio of Gram-negative bacteria to Gram-positive bacteria in each group was significantly higher in the model group than in the normal group (P <0.05), and lower in the baicalin low-dose group compared with the model group (P <0.05). 【Conclusion】 Baicalin can treat metabolic inflammation caused by high-fat diet, which may be related to the regulation of intestinal flora by baicalin.