黄芩苷对代谢性炎症及肠道菌群的调节作用探讨

来源 :广州中医药大学学报 | 被引量 : 0次 | 上传用户:chen19881220
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
【目的】探讨黄芩苷对高脂血症模型代谢性炎症及肠道菌群的调节作用。【方法】选取32只C57BL/6J雄性小鼠随机平均分为正常组、模型组、黄芩苷高剂量组(剂量为50 mg·kg-1·d-1)、黄芩苷低剂量组(剂量为25 mg·kg-1·d-1)。高脂饲料喂养3周后,2个黄芩苷用药组分别给予不同浓度黄芩苷灌胃5周。采用酶联免疫吸附法检测各组小鼠血清血脂水平以及肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)及内毒素水平,采用Miseq平台对小鼠肠道粪便微生物进行基因测序。【结果】与正常组比较,模型组总胆固醇及低密度脂蛋白水平显著升高(P<0.01),高密度脂蛋白水平显著降低(P<0.01),血清TNF-α、IL-6和内毒素水平显著升高(P<0.01);黄芩苷高剂量组的TNF-α、IL-6、内毒素水平均较模型组显著降低(P<0.05或P<0.01),黄芩苷低剂量组内毒素水平亦较模型组显著降低(P<0.01)。测序生物信息学分析发现,在门的水平,模型组脱铁杆菌门和变形杆菌门数目较正常组显著增多,疣微菌门数目较正常组显著减少。在属的水平,模型组Christensenella、uncultured_Peptococcaceae、脱硫弧菌属数目较正常组显著增多,而粪球菌属、Akkermansia及uncultured_Lachnospiraceae数目较正常组显著减少;黄芩苷高、低剂量组脱硫弧菌属丰度均较模型组降低。各组革兰氏阴性菌与革兰氏阳性菌数目之比,模型组较正常组显著升高(P<0.05),黄芩苷低剂量组较模型组显著降低(P<0.05)。【结论】黄芩苷能治疗高脂饮食引起的代谢性炎症,这可能与黄芩苷能调节肠道菌群的结构有关。 【Objective】 To investigate the regulatory effect of baicalin on metabolic inflammation and intestinal microflora in hyperlipidemia model. 【Methods】 Thirty-two C57BL / 6J male mice were randomly divided into normal group, model group, baicalin high-dose group (50 mg · kg-1 · d-1), low dose baicalin group 25 mg · kg -1 · d -1). Three weeks after the high-fat diet was fed, the two baicalin groups were given different concentrations of baicalin respectively for 5 weeks. The levels of serum lipids, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and endotoxin in mice were detected by enzyme-linked immunosorbent assay (ELISA) Microorganism gene sequencing. 【Results】 Compared with the normal group, the level of total cholesterol and low density lipoprotein in the model group was significantly increased (P <0.01), the level of high density lipoprotein was significantly decreased (P <0.01), the level of serum TNF-α, IL- The levels of TNF-α, IL-6 and endotoxin in the baicalin high-dose group were significantly lower than those in the model group (P <0.05 or P <0.01) Toxins were also significantly lower than the model group (P <0.01). Sequencing bioinformatics analysis showed that at the level of the door, the number of the number of the de-esobacterium and the proteobacteria in the model group was significantly higher than that in the normal group, and the number of the group of the number of the wartime-type bacteria was significantly decreased compared with the normal group. At the genus level, the number of the group of Christensenella, uncultured_Peptococcaceae and the number of desulfovibrio in the model group increased significantly compared with the normal group, while the number of the genus Faecium, Akkermansia and uncultured_Lachnospiraceae was significantly decreased compared with the normal group; Lower than the model group. The ratio of Gram-negative bacteria to Gram-positive bacteria in each group was significantly higher in the model group than in the normal group (P <0.05), and lower in the baicalin low-dose group compared with the model group (P <0.05). 【Conclusion】 Baicalin can treat metabolic inflammation caused by high-fat diet, which may be related to the regulation of intestinal flora by baicalin.
其他文献
应用广义α-凹算子的不动点理论,得到了保证方程正解的存在和唯一性的标准,同时研究了方程解对参数的依赖性,研究了一类分数阶微分方程非局部边值问题正解的存在唯一性.
伴随我国综合实力的不断提升,市场竞争愈加激励,如何进一步提高单位发展水平显得尤为重要。在人力资源管理中,绩效机制是对员工的评价与激励,是增强单位活力和竞争力的重要管
2014年9月4日,国务院颁布《关于深化考试招生制度改革的实施意见》明确提出要深化高考考试内容改革,2015年起增加使用全国统一命题试卷的省份.2014年,使用全国统一命题试卷的
在各级各类的数学竞赛中经常出现含有根式的递推数列的试题,这类题往往结构复杂,方法多变而成为竞赛的难点,本文对此类题作一些探究和分析.
中国著名林学家、林业教育家李相符同志是北京林业大学(原北京林学院)首任院长。他主持学校工作的10年期间,正处在万事开头难的建校初期,他兢兢业业,带领全校师生克服基础设
知识经济的到来和经济全球化的发展,教育和经济的关系愈来愈密不可分,教育为经济服务,经济促进教育发展.教育,尤其是高等教育为社会经济、为区域经济发展提供智力并带来动力,
<正>1胶片固定架的改进在大批量焊接产品的射线照相检验过程中,每次需暗室处理的胶片很多,最好采用胶片自动冲洗机.但因国外产品价格昂贵,国内产品质量不太过关等原因,在线检
我在山上,外公在山上。我们都在山上的小田地里。山上只有我们。已是黄昏,太阳迟迟地坐在山窝窝处。它有些坐不稳,但仍勉强地抓着两旁的山峰。一大片一大片的黄晕,将薄薄的天
<正> 在管道焊缝的超声检验中,大量的足带有余高的超声波检验,但随着高深技术的发展与引进,越来越多的管道焊缝要求将余高铲平。我公司安装过的动力管道中主要有以下两种情形
写作是认识世界,认识自我,进行创造性表述的过程。很多同学怕写作,其实是因为不得法。不信,与我一起来研读《香菱学诗》,看香菱是怎样由不会学到精彩的。