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以内毒素（ＬＰＳ）和血小板激活因子（ＰＡＦ）刺激后枯否细胞（ＫＣ）膜磷酸肌醇转化、细胞内钙离子（［Ｃａ２＋］ｉ）反应及前列腺素Ｅ２（ＰＧＥ２）含量为指标，探讨了老化对ＫＣ膜磷脂功能的影响及维生素Ｅ（ＶＥ）的保护作用。结果：２４月龄ＫＣＰＧＥ２基础分泌能力和受中、高浓度ＬＰＳ刺激后的ＰＧＥ２分泌能力均明显低于６月龄组；１８和２４月龄组ＫＣ［Ｃａ２＋］ｉ基础水平显著高于６月龄组；１８和２４月龄ＫＣ基础总磷酸肌醇含量明显低于６月龄组，各组ＫＣ受ＬＰＳ刺激后总磷酸肌醇含量增加不明显，受ＰＡＦ刺激后明显增加，但１８和２４月龄ＫＣ的增值明显小于６月龄组。ＶＥ处理组（ＶＥＧ）ＫＣＰＧＥ２基础分泌能力和总磷酸肌醇含量均明显高于非ＶＥ处理组（ＮＶＥＧ），２４月龄ＶＥＧ组ＫＣ受ＰＡＦ刺激后明显高于ＮＶＥＧ。１８和２４月龄ＶＥＧ［Ｃａ２＋］ｉ基础水平明显低于ＮＶＥＧ，活化后其净增值显著高于ＮＶＥＧ。提示，老化ＫＣ膜磷脂功能明显异常，ＶＥ对其具有显著的保护作用。
The changes of phosphoinositide conversion, intracellular Ca2 + ([Ca2 +] i) and prostaglandin E2 (PGE2) content in Kupffer cell (KC) cells stimulated by endotoxin (LPS) and platelet activating factor Effect of Aging on the Function of KC Phospholipid and the Protective Effect of Vitamin. Results: The basal secretion of KCPGE2 at 24 months of age and the secretion of PGE2 by LPS at moderate and high concentrations were significantly lower than those at 6 months. The basal levels of KC [Ca2 +] i at 18 and 24 months were significantly higher than those at 6 months Group; The content of total phosphoinositides of KC in 18th and 24th month was significantly lower than that in 6th month. The total inositol phosphate content of KC in each group was not obviously increased after stimulated by LPS, The added value of age KC was significantly less than that of 6 month old group. The basal secretion capacity and total inositol phosphate content of KCPGE2 in VE group were significantly higher than those in non-VE group (P <0.05). The 24-month-old KC group was significantly higher than NVEG after stimulated by PAF. The basal levels of VEG [Ca2 +] i at 18 and 24 months were significantly lower than those at NVEG, and their net added value after activation was significantly higher than that of NVEG. Tip, aging KC membrane phospholipids significantly abnormal function, VE has a significant protective effect.