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目的:探讨大黄酸(rhein)对血管紧张素Ⅱ(angiotensin,AngⅡ)诱导的大鼠近端肾小管上皮细胞肥大的影响。方法:麻醉下无菌分离SD大鼠单根近球小管并培养,以免疫细胞化学方法鉴定体外培养的肾小管上皮细胞。AngⅡ(10-7mol·L-1)培养肾小管上皮细胞,与此同时,加入不同剂量的大黄酸,作用72h后检测细胞体积、3H-亮氨酸掺入量以及蛋白质含量以观察细胞肥大的变化。结果:AngⅡ培养72h导致细胞体积明显增大,3H-亮氨酸掺入量及细胞内蛋白质含量明显增加。加入大黄酸处理72h后,大黄酸可明显降低AngⅡ所致的细胞体积增大,明显降低3H-亮氨酸掺入量及细胞内蛋白质含量的升高。结论:大黄酸可抑制血管紧张素Ⅱ诱导的肾小管上皮细胞肥大。
Objective: To investigate the effect of rhein on angiotensin II (Ang II) induced proximal tubular epithelial cell hypertrophy in rats. METHODS: Single rat proximal tubules of SD rats were aseptically isolated under anesthesia and cultured. The tubular epithelial cells cultured in vitro were identified by immunocytochemistry. Renal tubular epithelial cells were cultured with AngII (10-7 mol·L-1). At the same time, different doses of rhein were added. After 72 hours, the cell volume, 3H-leucine incorporation, and protein content were measured to observe the cellular hypertrophy. Variety. RESULTS: AngII cultured for 72 h resulted in a significant increase in cell volume, 3H-leucine incorporation, and intracellular protein content. After being treated with rhein for 72 h, Rhein can significantly reduce the increase of cell volume caused by AngII, and significantly reduce the increase of 3H-leucine incorporation and intracellular protein content. CONCLUSION: Rhein can inhibit angiotensin II-induced renal tubular epithelial cell hypertrophy.