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目的:探讨微小RNA（miR）-101靶向调节锌指E盒增强子结合蛋白1（ZEB1）对胃癌细胞上皮间质转化（EMT）及化疗敏感性的影响。方法:体外培养人胃癌MGC-803细胞，分为对照组（不做处理）、顺铂组（8 μmol/L顺铂）、mimic NC组（8 μmol/L顺铂+转染negative control-miR-101）、miR-101 mimic组（8 μmol/L顺铂+转染hsa-miR-101 mimic）。实时荧光定量PCR（qRT-PCR）法检测各组MGC-803细胞中miR-101、ZEB1、E-钙黏蛋白（E-cadherin）、N-钙黏蛋白（N-cadherin）、波形蛋白（Vimentin）、纤连蛋白（Fibronectin）mRNA表达情况；双荧光素酶报告基因实验验证miR-101与ZEB1的靶向作用关系；蛋白印迹（WB）法检测各组MGC-803细胞中相关蛋白表达情况；CCK-8法检测各组MGC-803细胞增殖情况；流式细胞仪检测各组MGC-803细胞凋亡情况。结果:与对照组相比，顺铂组MGC-803细胞中miR-101、E-cadherin mRNA及蛋白表达水平、细胞凋亡率显著升高[（1.01±0.09）vs（1.25±0.08），（1.00±0.10）vs（1.37±0.12），（0.26±0.04）vs（0.49±0.07），（6.85±0.95）% vs（15.44±1.12）%，（n P<0.05）]，ZEB1、N-cadherin、Vimentin、Fibronectin mRNA及蛋白表达水平、细胞OD值显著降低[（1.02±0.10）vs（0.82±0.08）,（1.02±0.08）vs（0.73±0.09）,（0.98±0.09）vs（0.79±0.09）,（0.99±0.08）vs（0.66±0.09）,（0.59±0.06）vs（0.30±0.05），（n P<0.05）]；与mimic NC组相比，miR-101 mimic组MGC-803细胞中miR-101、E-cadherin mRNA及蛋白表达水平、细胞凋亡率显著升高[（1.26±0.08）vs（1.57±0.10），（1.35±0.11）vs（1.66±0.11），（0.51±0.09）vs（0.68±0.09），（16.12±1.05）% vs（39.97±1.84）%，（n P<0.05）]，ZEB1、N-cadherin、Vimentin、Fibronectin mRNA及蛋白表达水平、细胞OD值显著降低[（0.84±0.08）vs（0.65±0.07），（0.76±0.09）vs（0.55±0.08），（0.75±0.08）vs（0.61±0.07），（0.65±0.07）vs（0.47±0.07），（0.31±0.05）vs（0.22±0.04）（n P<0.05）]。n 结论:miR-101可能通过靶向抑制ZEB1表达，抑制MGC-803细胞EMT过程，并提高癌细胞对化疗药物顺铂的敏感性。“,”Objective:To investigate the effects of microRNA (miR) -101 targeting zinc-finger E-box binding homeobox 1 (ZEB1) on epithelial-mesenchymal transition (EMT) and chemosensitivity of gastric cancer cells.Methods:Human gastric cancer MGC-803 cells were cultured in vitro and divided into control group (without treatment) , cisplatin group (8 μmol/L cisplatin) , mimic NC group (8 μmol/L cisplatin + negative control-miR-101) and miR-101 mimic group (8 μmol/L cisplatin + hsa-miR-101 mimic) . The expressions of miR-101, ZEB1, E-cadherin, N-cadherin, Vimentin and Fibronectin mRNAs in MGC-803 cells were detected by real-time fluorescent quantitative PCR (qRT-PCR) ; the double luciferase reporter gene experiment was used to confirm the targeting relationship between miR-101 and ZEB1; the expressions of related proteins in MGC-803 cells were detected by Western blot; the proliferation of MGC-803 cells was detected by CCK-8 method; and the apoptosis of MGC-803 cells was detected by flow cytometry.Results:Compared with those in the control group, the mRNA and protein expression levels of miR-101 and E-cadherin, and apoptosis rate of MGC-803 cells in cisplatin group were significantly higher (n P<0.05) , and the mRNA and protein expression levels of ZEB1, N-cadherin, vimentin and fibronectin, cell OD value were significantly lower (n P<0.05) ; meanwhile, compared with those in mimic NC group, the mRNA and protein expression levels of miR-101 and E-cadherin, and apoptosis rate of MGC-803 cells in miR-101 mimic group were significantly higher (n P<0.05) , and the mRNA and protein expression levels of ZEB1, N-cadherin, vimentin and fibronectin, cell OD value were significantly lower (n P<0.05) .n Conclusion:MiR-101 may inhibit the EMT process of MGC-803 cells and increase the sensitivity of cancer cells to cisplatin by targetingly inhibiting the expression of ZEB1.