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目的:研究金荞麦黄酮对2型糖尿病(T2DM)小鼠糖脂代谢及体内氧化应激作用。方法:昆明种小鼠喂食高脂饲料结合一次性腹腔注射40 mg/kg的链脲佐菌素建立糖尿病小鼠模型。将成模小鼠随机分为模型对照组、二甲双胍组(75 mg/kg)、金荞麦黄酮组(50、100、200mg/kg),另设正常对照组。观察给药期间各组小鼠体重和空腹血糖(FBG)变化。给药6周进行糖耐量实验,计算糖耐量曲线下面积(area under the curve,AUC);然后小鼠眼球取血,检测血清中胰岛素水平(FINS)、甘油三酯(TG)、胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、超氧化物歧化酶(SOD)、丙二醛(MDA)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px),并计算胰岛素抵抗指数(HOMA-IR)和胰岛素敏感指数(ISI)。结果:与正常对照组比较,模型对照组小鼠体重减轻,FBG、FINS、糖耐量、TG、TC、LDL-C、MDA、HOMA-IR升高,HDL-C、SOD、CAT、GSH-Px、ISI下降。与模型对照组比较,金荞麦黄酮在50~200mg/kg剂量范围内可不同程度回调上述指标,且金荞麦黄酮(100、200mg/kg)剂量组作用效果更为显著。结论:金荞麦黄酮在50~200mg/kg剂量范围内对T2DM小鼠有降血糖作用,其作用机制与调节血脂代谢和抗氧化作用有关。
Objective: To investigate the effects of buckwheat flavonoids on glucose and lipid metabolism and oxidative stress in type 2 diabetic (T2DM) mice. Methods: Kunming mice were fed with high-fat diet combined with a single intraperitoneal injection of streptozotocin (40 mg / kg) to establish a diabetic mouse model. Mice were randomly divided into model control group, metformin group (75 mg / kg), buckwheat flavonoids group (50,100,200 mg / kg), another set of normal control group. The changes of body weight and fasting blood glucose (FBG) in each group were observed during the administration. After 6 weeks of administration, the glucose tolerance test was performed to calculate the area under the curve (AUC) of the blood glucose level. Blood was collected from the eyeball of the mice and the level of insulin (FINS), triglyceride (TG), cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT) Glycometabolide peroxidase (GSH-Px), and calculated insulin resistance index (HOMA-IR) and insulin sensitivity index (ISI). Results: Compared with the normal control group, the body weight, FBG, FINS, glucose tolerance, TG, TC, LDL-C, MDA and HOMA- , ISI decline. Compared with the model control group, buckwheat flavonoids in the dose range of 50 ~ 200mg / kg can be adjusted to varying degrees above indicators, and buckwheat flavonoids (100,200mg / kg) dose group effect is more significant. Conclusion: Buckwheat flavone has hypoglycemic effect on T2DM mice in the dose range of 50 ~ 200mg / kg, and its mechanism is related to the regulation of blood lipid metabolism and antioxidation.