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目的:建立微分脉冲伏安法测定注射用头孢哌酮钠他唑巴坦钠中头孢哌酮钠含量的方法。方法:分析复方制剂在电化学循环伏安法和微分脉冲法下的不同伏安行为,确定最佳实验条件和测定方法。结果:在250 mmol·L-1磷酸二氢钠缓冲液中他唑巴坦钠没有响应,头孢哌酮钠检测质量浓度在13.7～171.4 mg·L-1与其峰电流值呈良好的线性关系(r=0.999 4),最低检测限为2.58 mg·L-1,回收率(n=6,RSD 1.67%)97.8%～102.3%。结论:该方法可有效消除他唑巴坦钠对测定头孢哌酮钠的干扰,可用于注射用头孢哌酮钠他唑巴坦钠中头孢哌酮钠的含量测定。灵敏度高,耗材少,重复性可靠。
Objective: To establish a differential pulse voltammetry method for the determination of cefoperazone sodium in cefoperazone sodium and tazobactam sodium for injection. Methods: The different voltammetric behavior of compound preparations under electrochemical cyclic voltammetry and differential pulse method was analyzed to determine the best experimental conditions and determination methods. Results: Tazobactam sodium did not respond in 250 mmol·L-1 sodium dihydrogen phosphate buffer solution. The concentration of cefoperazone sodium in the range of 13.7-171.4 mg · L-1 showed a good linear relationship with the peak current (r = 0.999 4). The lowest limit of detection was 2.58 mg · L-1. The recoveries (n = 6, RSD 1.67%) ranged from 97.8% to 102.3%. Conclusion: This method can effectively eliminate the interference of tazobactam sodium for the determination of cefoperazone sodium, which can be used for the determination of cefoperazone sodium in cefoperazone sodium and tazobactam sodium for injection. High sensitivity, less consumables, repeatable and reliable.