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目的探讨出生后早期接触狄氏剂对ICR小鼠哺乳期、青春期和成年期海马突触发育的影响。方法出生后小鼠随机分为5组,分别为0.2、2和20μg/kg 3个狄氏剂染毒组及溶剂对照组和生理盐水组,于出生后第3天(postnatal 3 days,PND 3)腹腔注射染毒,隔天1次,连续6次,分别在哺乳期(PND 14)、青春期(PND 36)和成年期(PND 98)分离海马。采用蛋白质印迹法检测海马突触前标志SynapsinⅠ和突触后标志突触后致密蛋白95(postsynaptic density protein 95,PSD 95)等突触相关蛋白水平。结果雌性小鼠高剂量染毒组SynapsinⅠ的蛋白水平在3个阶段均明显下降(均有P<0.05),但雄性各组间差异均无统计学意义;雌性小鼠青春期狄氏剂染毒中、高剂量组PSD 95均出现下降,并且存在剂量依赖性(rPND 36=-0.822,P中=0.034;P高=0.031),到成年期,各染毒剂量组PSD 95蛋白水平均下降,且存在剂量依赖关系(rPND 36=-0.743,PPND 14=0.015,PPND 36=0.032;PPND 98=0.027)。同时,雄性小鼠高剂量染毒组PSD 95蛋白水平在3个阶段均有下降(均有P<0.05)。结论出生后早期接触狄氏剂,对小鼠海马突触蛋白的表达产生持续影响,且存在明显的性别差异,雌性更加敏感。
Objective To investigate the effect of early postnatal dieldrin on the development of hippocampal synapses in lactating, adolescent and adulthood of ICR mice. Methods After birth, the mice were randomly divided into 5 groups: 0.2, 2 and 20 μg / kg of 3 dieldrin exposure groups and solvent control group and saline group. On the 3rd postnatal day (PND 3 ) Were injected intraperitoneally once a day for 6 consecutive times to separate the hippocampus during lactation (PND 14), adolescence (PND 36) and adulthood (PND 98). Western blotting was used to detect synapse-related proteins such as synapsin I and postsynaptic density 95 (PSD 95). Results The protein level of Synapsin Ⅰ in high dose exposure group decreased significantly in all three stages (all P <0.05), but there was no significant difference between male and female groups , And PSD 95 in high dose group decreased in a dose-dependent manner (rPND 36 = -0.822, P = 0.034; P = 0.031). In adulthood, There was a dose-dependent (rPND 36 = -0.743, PPND 14 = 0.015, PPND 36 = 0.032; PPND 98 = 0.027). At the same time, the level of PSD-95 in high-dose male mice decreased in three stages (all P <0.05). Conclusions Early postnatal exposure to dieldrin exerts a sustained effect on the expression of hippocampal synaptic proteins in mice, with significant gender differences and females being more sensitive.