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目的研究胃癌和食管癌循环肿瘤细胞(CTCs)的形态学特征。方法采用CTCBIOPSY异常细胞分离染色仪富集4例食管癌和7例胃癌患者外周血CTCs,依据本课题组拟定的CTCs形态学诊断标准对其进行鉴定,选取典型的CTCs,对其形态学(细胞学)特征进行研究。结果对典型的胃癌和食管癌CTCs的细胞形态学特征进行了初步描述,同时应用免疫荧光技术对检测到的CTCs进行了验证,结果检出CTCs的CK8/18/19(+),CD45(-),Hoechst(+),考虑为典型TCTs。结论本课题组拟定的CTCs形态学诊断标准科学可行;除形态学研究外,还需对筛选出的CTCs进行定性分析,以验证CTCs的性质,为其下游更为深入的研究提供基础。
Objective To study the morphological characteristics of gastric cancer and esophageal cancer circulating tumor cells (CTCs). Methods CTCs were collected from 4 patients with esophageal cancer and 7 patients with gastric cancer by CTCBIOPSY Abnormal Cell Separation Staining Instrument. According to the diagnostic criteria of CTCs formulated by our group, typical CTCs were selected and their morphology Cytology) characteristics of the study. Results The morphological features of typical CTCs in gastric cancer and esophageal cancer were preliminarily described. At the same time, the CTCs detected by immunofluorescence were validated. The results showed that CK8 / 18/19 (+) and CD45 (- ), Hoechst (+), considered as typical TCTs. Conclusions In addition to morphological studies, the CTCs morphological diagnostic criteria developed by our group are scientifically feasible. Qualitative analysis of the selected CTCs is also required to verify the properties of CTCs and provide a basis for further studies on their CTCs.