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叉头框M1(forkhead box M1,FOXM1)是一种与细胞增殖相关的转录因子,对各种恶性肿瘤的发生以及发展起促进作用。有相关研究表明FOXM1在肝细胞肝癌(hepatocellular carcinoma,HCC)中高表达,但其与患者临床病理特征的关系以及对患者预后的影响并未报道。我们采用免疫组织化学染色方法检测FOXM1在HCC组织(38例)及配对癌旁组织(10例)中的表达情况,结果显示FOXM1高表达于癌组织中而不表达于癌旁组织中,同时更明显表达于低分化癌组织中。采用Fisher确切概率法分析HCC组织中FOXM1表达与患者肝功能指标及临床病理参数的关系,结果表明FOXM1仅与癌组织的分化程度相关,而与HCC患者肝功能指标及其他病理特征不相关。同时,我们还采用Kaplan-Meier生存曲线及Cox多因素比例风险回归模型分析FOXM1与HCC患者预后的相关性,结果表明FOXM1对HCC患者预后并无影响。我们还发现抑制FOXM1表达能够下调肝癌细胞株分泌AFP水平。这些结果提示我们,FOXM1可以作为一种鉴定HCC组织分化程度的生物学标志物,并且抑制FOXM1表达能够下调HCC患者AFP的分泌水平,然而并不能作为评估患者预后的指标。
Forkhead box M1 (FOXM1) is a transcription factor associated with cell proliferation that promotes the development and progression of various malignancies. Some studies have shown that FOXM1 is highly expressed in hepatocellular carcinoma (HCC), but its relationship with the clinicopathological features and prognosis of patients has not been reported. We detected the expression of FOXM1 in HCC tissues (38 cases) and adjacent tissues (10 cases) by immunohistochemical staining. The results showed that FOXM1 was highly expressed in cancerous tissues but not in adjacent tissues Obviously expressed in poorly differentiated carcinoma tissues. Fisher exact test was used to analyze the relationship between FOXM1 expression and liver function and clinicopathological parameters in HCC. The results showed that FOXM1 was only related to the degree of differentiation of HCC tissue, but not to liver function and other pathological features. At the same time, we also used Kaplan-Meier survival curve and Cox multivariate proportional hazard regression model analysis of FOXM1 and HCC patients prognosis, the results show that FOXM1 has no effect on the prognosis of patients with HCC. We also found that inhibition of FOXM1 expression downregulated AFP levels in hepatocellular carcinoma cell lines. These results suggest that FOXM1 can be used as a biomarker to identify the degree of HCC tissue differentiation and that inhibiting FOXM1 expression can down-regulate the secretion of AFP in HCC patients. However, FOXM1 can not be used as an indicator to evaluate the prognosis of HCC patients.