流行性出血热病毒(HFV)在人体的复制状况及其致病机理,目前还不十分清楚。现代分子生物学技术的发展,为此项研究提供了新手段。近年发展起来的非同位素标记探针—地高辛(DIG)酶联免疫系统是一种比较新的技术,敏感性高,标记方便,已广泛应用于病毒研究领域。我们采用DIG标记的HFV cDNA探针,并根据原位杂交 It is not yet clear whether the epidemic hemorrhagic fever virus (HFV) is replicating in humans and its pathogenesis. The development of modern molecular biology technology provides a new method for this research. In recent years, the developed non-isotope labeled probe - digoxin (DIG) enzyme-linked immunosorbent system is a relatively new technology, high sensitivity, convenient labeling, has been widely used in the field of virus research. We use DIG-labeled HFV cDNA probes and hybridize in situ