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目的建立玄蓝咽喉炎颗粒中黄芩苷和芍药苷的测定方法。方法采用双波长HPLC法,色谱柱:SHIMADZU VP-ODS(5μm,250 mm×4.6 mm);流动相:甲醇-0.05 mol·L~(-1)磷酸二氢钾溶液(38:62);流速1.0 mL·min~(-1);柱温:30℃;检测波长:1~10 min,230 nm;10~35 min,280 nm。结果黄芩苷在29.98~599.73μg·mL~(-1)浓度范围、芍药苷在7.99~159.83μg·mL~(-1)浓度范围线性关系良好;黄芩苷的平均回收率为98.74%,RSD为0.5%;芍药苷的平均回收率为98.68%,RSD为1.5%。结论该方法简便,重复性好,可为玄蓝咽喉炎颗粒的质量控制提供参考。
Objective To establish a method for the determination of baicalin and paeoniflorin in Xuan Lan pharyngitis granules. Methods The dual wavelength HPLC method was used. The chromatographic column was SHIMADZU VP-ODS (5 μm, 250 mm × 4.6 mm). The mobile phase consisted of methanol-0.05 mol·L -1 potassium dihydrogen phosphate solution (38:62) 1.0 mL · min -1; column temperature: 30 ℃; detection wavelength: 1 ~ 10 min, 230 nm; 10 ~ 35 min, 280 nm. Results The concentration of paeoniflorin in the range of 29.98 ~ 599.73μg · mL ~ (-1) was good. The average recovery of baicalin was 98.74% 0.5%. The average recovery of paeoniflorin was 98.68% with RSD of 1.5%. Conclusion The method is simple and reproducible, which can provide reference for the quality control of Xuan Lan Pharyngitis Granules.