论文部分内容阅读
目的探讨1-溴丙烷(1-BP)对中国仓鼠肺细胞(V79)次黄嘌呤鸟嘌呤磷酸核糖转移酶(hprt)基因位点的致突变作用。方法将V79分为大鼠肝微粒体混合功能氧化酶(S9)代谢活化系和非S9代谢活化系,每系各设3.375、6.750、13.500 g/L 3个不同质量浓度的1-BP实验组;另设1.000 g/L甲磺酸乙酯阳性对照组(非S9代谢活化系)、0.001g/L甲基硝基亚硝基胍阳性对照组(S9代谢活化系)和溶剂对照组,溶剂对照组加等体积的无血清培养基。染毒时间为6 h。应用细胞克隆检测法检测V79 hprt基因位点的突变率。结果非S9代谢活化系,1-BP实验组突变率与溶剂对照组比较,差异均无统计学意义(P>0.05);S9代谢活化系,3.375、6.750 g/L 1-BP实验组突变率均高于溶剂对照组,差异均有统计学意义(P<0.01),但不存在剂量-反应关系。结论 1-BP经代谢活化后可能对V79 hprt基因位点具有致突变作用。
Objective To investigate the mutagenic effects of 1-bromopropane (1-BP) on the hypoxanthine guanine phosphoribosyltransferase (hprt) gene in Chinese hamster lung cells (V79). Methods V79 was divided into metabolic activation system (S9) and non-S9 metabolic activation system in rat liver microsomes. Each experimental group consisted of 3 groups of 1-BP with different concentrations of 3.375, 6.750 and 13.500 g / L ; Another set of 1.000 g / L ethyl methanesulfonate positive control group (non-S9 metabolic activation), 0.001g / L methyl nitrosoguanidine positive control group (S9 metabolic activation) and solvent control group, solvent The control group plus an equal volume of serum-free medium. Exposure time is 6 h. The mutation rate of V79 hprt gene locus was detected by cell clone assay. Results There were no significant differences in the mutation rates between the non-S9 metabolic activation group and the 1-BP experimental group and the solvent control group (P> 0.05). The mutation rate of S9 metabolic activation group, 3.375,6.750 g / L 1-BP experimental group Higher than the solvent control group, the difference was statistically significant (P <0.01), but there is no dose-response relationship. Conclusion The metabolic activation of 1-BP may cause mutagenesis of V79 hprt locus.