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柔红霉素(DR)可引起心肌毒性损害和明显的白细胞减少而限制了它的应用。心肌组织缺乏内摄能力,用脂质体作柔红霉素载体有可能防止对心肌组织的损害。本实验报告给小鼠游离和脂质休包埋的DR后,有关不同血清酶的改变,组织内DR水平以及心肌损伤情况。脂质体为卵磷脂胆硷(PC)、胆固醇(CH)、二鲸腊磷酸脂(DCP)或硬脂酰胺(SA)脂溶液(100mg/ml),克分子比例分别为7:3:1,经旋转蒸发.,真空干燥,将干燥脂质薄膜溶于含DR(DR:PC=1:15为带负电荷的微小单层颗粒型,-SUV;1:30为+SUV)的Tris缓冲液中,经一系列处理后,脂质体悬液中带负荷的DCP脂质体含DR为1.0—1.5mg/ml,带正电荷的SA含DR为0.2—0.4mg/ml。游离与包埋的DR用量为20或35mg/kg,容量为0.35—0.59ml/每只鼠静注。结果表明,给药后1、3、6天分
Daunorubicin (DR) limits its use by causing cardiotoxic damage and significant leukopenia. Myocardial tissue lack of endocrine capacity, with daunorubicin liposomes carrier may prevent damage to myocardial tissue. This experiment report to the mouse free and lipid-embedded DR, the changes of different serum enzymes, the level of tissue DR and myocardial injury. Liposomes were lecithin (PC), cholesterol (CH), bis-whale phosphatidylinositol (DCP) or stearamide (SA) lipid solution (100mg / ml) in molar ratios of 7: 3: 1 , Vortexed, vacuum dried, and the dried lipid film was dissolved in Tris buffer containing DR (DR: PC = 1: 15 as negatively charged microparticulate, -SUV; 1:30 as + SUV) After a series of treatments, the loaded DCP liposomes in the liposomal suspension contained a DR of 1.0-1.5 mg / ml and the positively charged SA-containing DR 0.2-0.4 mg / ml. Free and embedded DR dose of 20 or 35mg / kg, a capacity of 0.35-0.59ml / per mouse intravenously. The results showed that 1, 3, 6 days after administration