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G1到S期的转换是植物细胞周期中一个关键的调控点,而D型细胞周期蛋白(CYCD)在这一转换过程中起着重要作用。CYCD通过感受外界信号的刺激,调控细胞周期进程,进而影响植物的生长发育。为研究木本植物中不同CYCD基因家族的功能,从黑杨中克隆出6个CYCD基因,并将其转化至酵母G1期细胞周期蛋白突变体进行功能鉴定。各家族CYCD基因均能对酵母突变体进行回补,但回补后促进酵母生长的能力存在差异。通过对黑杨组培苗进行糖和植物激素处理,观察到黑杨根部形态发生改变,同时用实时荧光定量PCR技术检测了该处理条件下CYCD基因表达量的变化。结果表明,各CYCD基因家族的代表成员对糖和植物激素的响应不同,反映了不同黑杨CYCD基因在树木生长发育过程中所起作用的差异性。
The transition from G1 to S phase is a key regulatory point in plant cell cycle, and D-type cyclin (CYCD) plays an important role in this conversion process. CYCD through the feelings of the external signal stimulation, regulating cell cycle progression, thereby affecting plant growth and development. To study the function of different CYCD gene families in woody plants, six CYCD genes were cloned from Populus tomentosa and transformed into yeast G1-phase cyclin mutants for functional characterization. The CYCD genes of each family were able to replenish yeast mutants, but there was a difference in their ability to promote yeast growth after replenishment. Through the treatment of sugar and plant hormones on the tissue culture seedlings of Populus tomentosa, morphological changes of the root of Populus nigra were observed, and the changes of CYCD gene expression under the treatment conditions were detected by real-time fluorescence quantitative PCR. The results showed that the representatives of each CYCD gene family had different responses to sugars and phytohormone, which reflected the difference of CYCD genes in different Populus deltoids during their growth and development.