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目的探讨甲基转移酶(DNMT)活性在系统性红斑狼疮(SLE)患者外周血单个核细胞(PBMC)中的变化及其意义。方法分离SLE患者及正常人群PBMC,抽提核蛋白,采用DNMT活性检测试剂盒检测两组PBMC中的DNMT活性。对SLE患者的DNMT催化活性与系统性红斑狼疮疾病活动指数(SLE-DAI)进行相关分析。结果 SLE患者PBMC的DNMT活性较正常对照组明显下降,两组比较差异有统计学意义(P<0.05),但是与SLEDAI评分无明显相关性(P>0.05)。结论 SLE患者PBMC的DNMT活性明显下降,DNMT活性下降可能与SLE的发病相关。
Objective To investigate the changes of methyltransferase (DNMT) activity in peripheral blood mononuclear cells (PBMCs) in patients with systemic lupus erythematosus (SLE) and its significance. Methods PBMC were isolated from patients with SLE and normal controls. Nucleoprotein was extracted and the activity of DNMT in both PBMCs was detected by DNMT activity assay kit. The correlation between DNMT catalytic activity and systemic lupus erythematosus disease activity index (SLE-DAI) in patients with SLE was analyzed. Results The DNMT activity of PBMC in SLE patients was significantly lower than that in normal controls (P <0.05), but no significant correlation with SLEDAI score (P> 0.05). Conclusion The DNMT activity of PBMCs in patients with SLE is significantly decreased, and the decrease of DNMT activity may be related to the pathogenesis of SLE.