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目的 建立制备STR的等位基因梯阶标准对照的新方法。方法 以T-质粒载体直接连接STR等位基因扩增产物,导入大肠杆菌,使其随大肠杆菌的无性繁殖而复制、扩增,获得单一DNA分子的大量拷贝;经PCR鉴定及测序分析后,制备出标准的STR等位基因梯阶对照(AL)。结果 所建方法制备出了标准AL;保存重组质粒及转染的大肠杆菌,可保证AL的大量快速制备,并能长期保存。结论 该方法制备的AL在法医物证检验中有很高的应用价值,对STR试剂盒国产化有重要意义。
OBJECTIVE: To establish a new method for preparing STR allelic ladder standard control. Methods T-plasmid vector was used to directly ligate the amplified product of STR allele into Escherichia coli. The recombinant plasmid was introduced into Escherichia coli to clone and amplify the Escherichia coli. The PCR products were identified by PCR and sequenced. A standard STR allele ladder control (AL) was prepared. Results The standard AL was prepared. The recombinant plasmids and E. coli were transfected to ensure that a large amount of AL could be rapidly prepared and could be stored for a long time. Conclusion The AL prepared by this method has high value in the forensic evidence testing and is of great significance to the localization of STR kits.