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目的研究维生素E对H2O2引起的人甲状腺细胞凋亡的影响。方法取良性甲状腺腺瘤手术中的腺瘤旁正常甲状腺组织进行细胞培养。预防组加VE(50μmol/L)或无血清培养液后再加入不同浓度H2O(2200～800μmol/L),治疗组预先加入不同浓度H2O(2200～800μmol/L)后再加入VE或无血清培养液刺激单层培养的甲状腺细胞,MTT法检测细胞存活率,流式细胞术(FCM)检测各组甲状腺细胞凋亡率。结果预防组和治疗组单纯200～800μmol/L H2O2作用24 h均可使甲状腺上皮细胞存活率降低、凋亡率升高(P<0.01),并呈浓度效应关系。与相应单纯H2O2处理相比,加入50μmol/L VE预干预及治疗后,细胞存活率升高、凋亡率下降,400、800μmol/L H2O2处理时上述反应尤为显著(P<0.05)。结论维生素E对H2O2诱导的人甲状腺细胞凋亡具有保护作用。
Objective To investigate the effect of vitamin E on H2O2-induced apoptosis in human thyrocytes. Methods The normal thyroid tissue adjacent to the adenoma in benign thyroid adenoma was cultured for cell culture. The patients in the prevention group were treated with VE (50μmol / L) or serum-free medium and then added with different concentrations of H 2 O (2200-800μmol / L). The treatment group was pretreated with different concentrations of H 2 O (2200-800μmol / L) The monolayer culture of thyroid cells was stimulated by liquid, the cell viability was detected by MTT assay and the apoptosis rate of thyroid cells was detected by flow cytometry (FCM). Results After treated with 200 ~ 800μmol / L H2O2 for 24 h, both the prophylaxis group and the treatment group decreased the survival rate of thyroid epithelial cells and increased the apoptosis rate (P <0.01). Compared with the corresponding pure H2O2 treatment, pre-treatment and pretreatment with 50μmol / L VE increased the cell viability and decreased the apoptosis rate, especially at 400 and 800μmol / L H2O2 treatment (P <0.05). Conclusion Vitamin E has a protective effect on H2O2-induced apoptosis of human thyrocytes.