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在用沙门氏菌/微粒体试验系统研究无机化合物致突变性的过程中,发现六价铬(铬酸盐和重铬酸盐)被需还原型辅酶Ⅱ的微粒体酶系统所代谢,致使突变活性降低。加入一种完全的微粒体活化系统(S-9混合液),致突变性显著降低,而未加辅酶Ⅱ的不完全系统,致突变性未降低。这些结果表明,辅酶Ⅱ的存在,是微粒体系统中促使Cr(Ⅵ)代谢的主要因素。本实验未研究氧的作用。在水溶液中混合六价铬和还原型辅酶Ⅱ时,氧化和还原情况都未变。这表明微粒体系统中至少存在一种对反应所必
In the study of the mutagenicity of inorganic compounds using the Salmonella / microsomal test system, hexavalent chromium (chromate and dichromate) was found to be metabolized by the microsomal enzyme system of the reduced coenzyme II, resulting in a decrease of the mutagenic activity . Adding a complete microsomal activation system (S-9 mixture), mutagenicity was significantly reduced, without the addition of coenzyme Ⅱ incomplete system, mutagenicity was not reduced. These results indicate that the presence of Coenzyme II is a major factor in the metabolism of Cr (VI) in the microsomal system. The experiment did not study the role of oxygen. In the aqueous solution of mixed hexavalent chromium and reduced coenzyme Ⅱ, the oxidation and reduction have not changed. This indicates that there is at least one type of microsomal system necessary for the reaction