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研究中所使用的抗原为马来丝虫感染性幼虫(L_3)和成虫,在冰浴中匀浆30分钟后超声粉碎10Kcs,10分钟,再离心沉淀10,000rpm,10分钟,分离上清液,测定蛋白质含量,具体方法见Chandra等(1978)技术操作。按Chandra等(1978)的方法,受试者两前臂内侧分别注射上述两种抗原,并于晚间8～10时取指端血作20cmm厚血片查微丝蚴,部分地区的受试者,随机抽样,作粪便直接涂片查蠕虫卵和其他寄生虫卵。试验地区的选择:1.非流行区两处:一处是阿南特纳格(克什米尔境内),当地常年气温很低,不适宜蚊媒的孳生和发育,人们也从不外出,故无丝虫病,但因卫生条件很差,
The antigens used in the study were Malayi infective larvae (L_3) and adult worms. After homogenization in an ice bath for 30 minutes, 10Kcs were sonicated for 10 minutes and centrifuged again for 10,000rpm for 10 minutes. The supernatant was separated, Determination of protein content, the specific method, see Chandra et al. (1978) technical operation. According to the method of Chandra et al. (1978), the subjects were injected with the above two antigens on the medial forearm and the fingertip blood was taken at 8 to 10 o’clock in the evening to check the microfilariae in 20cmm thick slices. In some areas, Random sampling, for direct smear stool check worm eggs and other parasite eggs. Test area of choice: 1. Non-endemic areas in two places: one is Anantnag (Kashmir territory), the local perennial temperatures are low, not suitable for mosquito breeding and development, people never go out, so no filariasis Sick, but because of poor sanitation,