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目的 :研究Survivin基因在大肠癌中的表达及临床意义。方法 :应用反转录RT PCR方法 ,检测了 2 9例大肠癌组织中Survivin基因mRNA的表达。结果 :发现正常大肠黏膜组织中Survivin基因不表达 ,肿瘤组织中Survivin基因表达阳性率为 72 .4 % (2 1/ 2 9)。半定量分析结果显示 ,2 9例大肠癌组织中Survivin其表达的平均值为 0 .6 1± 0 .39。Survivin基因表达与淋巴结转移 ,肿瘤分期及远处转移有关。但与年龄 ,肿瘤大小 ,分化程度无关。结论 :提示Survivin基因在大肠癌病程中起重要的作用 ,对筛选大肠癌术后高危转移患者 ,加强随访诊治有一定参考意义
Objective: To study the expression of survivin gene in colorectal carcinoma and its clinical significance. Methods: The mRNA expression of Survivin in 29 cases of colorectal carcinoma was detected by RT-PCR. Results: Survivin gene was not found in normal colorectal mucosa. The positive rate of Survivin gene in tumor tissue was 72.4% (2 1/29). Semi-quantitative analysis showed that the average expression of Survivin in 29 cases of colorectal cancer was 0.61 ± 0.39. Survivin gene expression and lymph node metastasis, tumor stage and distant metastasis. But with age, tumor size, the degree of differentiation has nothing to do. Conclusion: It is suggested that Survivin gene plays an important role in the course of colorectal cancer. It has some reference value for screening patients with high-risk metastasis of colorectal cancer and enhancing the follow-up and treatment