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目的建立多药耐药人胃癌细胞裸鼠原位移植瘤模型并探讨移植瘤的耐药性。方法将人胃癌细胞株SGC7901和耐药细胞株SGC7901/VCR分别接种于两组裸鼠胃壁,用MTT法测定瘤细胞对阿霉素的耐药性及RTPCR技术测定其多药耐药MDR基因表达。结果MTT法测定IC50分别为098mg/L和578mg/L,与移植前细胞相比差异无显著性意义IC50分别为101mg/L和520mg/L;RTPCR测定MDR基因表达:SCG7901组表达阴性,SGC7901/VCR组表达阳性。结论耐药人胃癌细胞裸鼠原位移植瘤为研究胃癌多药耐药性提供了具有器官微环境的动物模型。
Objective To establish an orthotopic xenograft model of multidrug-resistant human gastric cancer cells in nude mice and investigate the drug resistance of xenografts. Methods Human gastric cancer cell line SGC7901 and drug-resistant cell line SGC7901/VCR were inoculated into the stomach of two groups of nude mice respectively. MTT assay was used to determine the drug resistance of adriamycin and RTPCR was used to determine the expression of multidrug resistance MDR gene. . Results The IC50 of MTT assay was 098mg/L and 578mg/L, respectively, and there was no significant difference compared with the pretransplantation cells. The IC50 values were 101mg/L and 520mg/L respectively. The expression of MDR gene was detected by RTPCR: the expression was negative in SCG7901 group. The VCR group expressed positive. Conclusion The orthotopic xenografts of resistant gastric cancer cells in nude mice provide an animal model with organ microenvironment for the study of multidrug resistance in gastric cancer.