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目的：分析精神分裂症患者血清32 kD蛋白条带组成成分，探讨患者血清蛋白表达情况。方法收集60例首次发作精神分裂症患者和58例健康对照者，采集血清样本，用7%聚乙二醇（polyethylene glycol， PEG）沉淀法进行蛋白处理，经十二烷基磺酸钠-聚丙烯酰胺凝胶电泳（sodium dodecyl sulfate polyacrylamide gel electrophoresis，SDS-PAGE）获得差异表达的32 kD蛋白条带，对此条带进行质谱检测以分析其蛋白组成。结果38例精神分裂症患者血清中出现32 kD差异蛋白表达条带，阳性率为63.3%，对照组均未发现该蛋白条带，两组阳性率具有统计学差异（P<0.01）。质谱分析显示，在分子量为30~35 kD范围内鉴定出14种蛋白：6种高频表达的蛋白包括cDNA编码的未知蛋白1和2、载脂蛋白A-1、纤胶凝蛋白同种型、补体因子H和聚集因子；表达频率较低的8种蛋白包括IgG H链、锌-α2-糖蛋白、肾高血压蛋白原酶、家族性载脂蛋白L1、胶原凝素-1-同种型、嘌呤核苷、膜联蛋白及cDNA编码的未知蛋白3。cDNA编码的3种未知蛋白分别与补体C4-B、β-2糖蛋白、红细胞膜蛋白带7蛋白高度相似。结论精神分裂症患者血清中存在未知的特异性32 kD蛋白条带，与健康对照者存在明显的蛋白表达差异。蛋白质谱分析发现32 kD蛋白条带主要由14种蛋白组成。“,”Objective To analyze the constituent of the 32 kD protein band and its expression in schizophrenia se?rum. Methods Sixty schizophrenia patients and 58 health controls were recruited. The serum samples were collected and precipitated with 7%PEG. The sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was used to ob?tain the abnormal 32 kD proteins band in patients. This protein band was cut and then analyzed using mass spectrometric technique. Results The 32 kD protein band was present in 38 schizophrenia patients but not in control and positive rate was 63.33%. The mass spectrometric analysis showed that 32 kD protein band contained 14 proteins ranging from 30 kD to 35 kD, including 6 high-frequency proteins (cDNA coded protein 1 and 2, Apolin protein A-1, Isoform 2 of ficolin-2, Complement factor H and clusterin) and 8 low-frequency proteins (IgG H chain, zinc-alphg-2-glycoprotein, fermitin,family apolin protein L-1, isoform 10 of collectin-1, purine nucleoside, anne xin and cDNA coded protein 3). Three cD?NA coded unknown proteins were highly similar to complement C4-B, β2-glycoprotein and erythrocyte band 7 integral membrane protein. Conclusion There is a unknown specific 32 kD protein that is consisted mainly of fourteen proteins in serum of schizophrenia.