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目的 了解RIHA ,Kado ,PCR法检测鼠疫菌Fra1抗原的敏感性及特异性 ,比较三种方法的优劣。方法 采用反向间接血凝抑制法 (RIHA)、Kado法及双重式聚合酶链 (Pla -F1-PCR)反应三种方法。结果 检测鼠疫菌Fra1抗原检出率分别为 10 0 %,93 . 5 %和 96. 7%,经配对资料的卡方检验 ,三者间无显著性差异 ( χ2 =2 . 0 ,P <0 . 0 5 )。结论 RIHA法仅适用于追溯诊断 ,质粒图谱在分子流行病学上具有重要意义 ,双重式聚合酶链法不但具有快速、特异、敏感等特性 ,而且还可用于非典型菌株的检测 ,可为静息期鼠疫的防治提供科学依据
Objective To understand the sensitivity and specificity of RIHA, Kado and PCR methods to detect Fra1 antigen in Yersinia pestis and compare the advantages and disadvantages of the three methods. Methods Three methods of RIHA, Kado and Pla-F1-PCR were used. Results The detection rates of Fra1 antigen of Y. pestis were 100%, 93.5% and 96.7% respectively. There was no significant difference among the three groups (χ2 = 2.0, P <0 . 0 5). Conclusion The RIHA method is only suitable for retrospective diagnosis. Plasmid mapping is of great importance in molecular epidemiology. Duplex PCR is not only rapid, specific and sensitive, but also can be used for the detection of atypical strains, Provide a scientific basis for the prevention and treatment of interest-bearing plague