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目的建立地苦胆涂膜剂中盐酸巴马汀的含量测定方法。方法采用HPLC法,用甲醇做溶剂超声提取,使用十八烷基硅烷键合硅胶为填充剂;甲醇-浓度为0.025 mol.L-1的磷酸缓冲溶液(PH)(体积比为50∶50)为流动相;检测波长为340 nm。结果方法线性关系良好,平均回收率为98.1%,RSD为1.3%。结论HPLC法可以用于测定地苦胆涂膜剂中盐酸巴马汀的含量。
Objective To establish a method for the determination of palmatine hydrochloride in gallbladder film. Methods HPLC method was used for the ultrasonic extraction with methanol as the solvent. The octadecylsilane-bonded silica gel was used as the filler; the methanol-phosphate buffer solution (PH) with the concentration of 0.025 mol.L-1 was used (50:50 by volume). The mobile phase; detection wavelength is 340 nm. Results The method has a good linear relationship with an average recovery of 98.1% and an RSD of 1.3%. Conclusion The HPLC method can be used to determine the content of palmatine hydrochloride in the film.