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为了解血管内皮细胞对肝素的反应,我们研究了标准肝素和低分子量肝素组分或肝素成分与培养的人体内皮细胞结合的关系.肝素与内皮细胞结合的特征标准肝素和低分子量肝素成分与内皮细胞的结合是一缓慢过程,需数小时才能达到饱和平衡.通过不同的标记物发现特异结合比率很高.这些标记物是~(125)碘-肝素;~3氚-乙酰肝素或者~(36)S-肝素.~3氚-乙酰肝素用来观察总的饱和曲线,而实验数据绘制的Scatchard图表明两组的结合部位不同:一组对人体脐静脉内皮细胞亲合力较弱(Kd=1.37±0.13μM);另一组亲合力较前者高10倍,即Kd=
To understand the response of vascular endothelial cells to heparin, we studied the relationship between standard heparin and low molecular weight heparin components or heparin components and cultured human endothelial cells. Characteristics of heparin binding to endothelial cells Standard heparin and low molecular weight heparin components and endothelium The binding of cells is a slow process that takes several hours to reach saturation equilibrium. The specific binding ratios found by different markers are high. These markers are ~125 iodine-heparin; ~3 oxime-heparin or ~(36) ) S-heparin.~3氚-heparan-heparin was used to observe the total saturation curve. The Scatchard plot of experimental data showed that the binding sites of the two groups are different: a group of human umbilical vein endothelial cells have weak affinity (Kd=1.37). ± 0.13 μM); the other group is 10 times higher than the former, ie Kd=