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建立了敏感性高、特异性强的地高辛素探针原位杂交技术,并用于肝病和肝癌分子病理学的研究。我们发现慢性乙型肝炎与肝轻微病变52例中43例,肝硬化6例中5例,12例肝癌中10例癌周组织,8例癌组织内可检测出HBV DNA。上述结果揭示了慢性HBV感染与肝癌的分子病理联系。结果还发现原位杂交与转移杂交或PCR检测的结果有较好的一致性。地高辛素探针原位杂交技术可广泛用于消化系疾病分子病理研究。
A highly sensitive and specific digoxigenin probe in situ hybridization technique has been established and used for the study of liver pathology and hepatocellular carcinoma molecular pathology. We found 43 cases of 52 cases of chronic hepatitis B and mild liver disease, 6 cases of 6 cases of liver cirrhosis, 10 cases of 12 cases of hepatocellular carcinoma, and 8 cases of cancer tissue can be detected in HBV DNA. The above results reveal the molecular pathological association between chronic HBV infection and liver cancer. The results also showed good agreement between in situ hybridization and transfer hybridization or PCR. Digoxigenin probe in situ hybridization can be widely used in molecular pathology of digestive diseases.