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AP2/EREBP家族的转录因子在调控植物生长发育和应答环境胁迫方面具有重要作用。利用同源克隆结合RACE(rapid-amplification of cDNA ends)技术,从四合木(Tetraena mongolica)中克隆了AP2/EREBP家族的基因,将其命名为TmAP2-1(GenBank登录号:JQ676996)。序列分析结果表明,该基因的开放阅读框长度为1452bp,编码483个氨基酸;比对结果显示TmAP2-1有2个AP2/ERF结构域,属于AP2/EREBP转录因子家族的AP2亚家族。亚细胞定位实验结果表明,TmAP2-1定位在细胞核中。该基因编码的蛋白在酵母中没有转录激活活性。利用Real-timePCR检测发现该基因在根、茎、叶等器官中均表达,且在叶中表达量最高。此外,TmAP2-1还受到NaCl、低温、PEG和ABA的强烈诱导,推测TmAP2-1可能参与四合木的逆境胁迫响应。在四合木愈伤组织中过表达该基因能够降低四合木愈伤组织中油脂的含量,同时提高可溶性糖的含量,暗示该基因可能通过影响糖代谢过程参与逆境胁迫响应。
The transcription factor of AP2 / EREBP family plays an important role in regulating plant growth and responding to environmental stress. The gene of AP2 / EREBP family was cloned from Tetraena mongolica by using rapid-amplification of cDNA ends (RACE) and named as TmAP2-1 (GenBank accession number: JQ676996). Sequence analysis showed that the open reading frame of this gene was 1452bp and encoded a protein of 483 amino acids. The alignment showed that TmAP2-1 has two AP2 / ERF domains and belongs to the AP2 subfamily of AP2 / EREBP transcription factor family. Subcellular localization experiments showed that TmAP2-1 was located in the nucleus. The gene encodes a protein that is not transcriptionally active in yeast. Real-time PCR showed that the gene was expressed in roots, stems, leaves and other organs, and the highest expression in leaves. In addition, TmAP2-1 was also strongly induced by NaCl, low temperature, PEG and ABA, suggesting that TmAP2-1 may be involved in the stress response of Tetraena mongolica. Overexpression of this gene in Siraitia callus could reduce the oil content in callus and increase the content of soluble sugar, suggesting that the gene may be involved in the response to stress by affecting glucose metabolism.