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背景:青风藤临床用于治疗阿片类戒断综合征有明显的疗效,但该药的作用及作用机制尚未明确。目的:探讨中药青风藤及其有效成分青藤碱对吗啡依赖动物模型催促戒断反应的影响。设计:完全随机对照实验研究。单位:解放军第一军医大学中医系。对象:体外实验研究对象为豚鼠离体回肠,分为正常回肠对照组,吗啡依赖回肠组,青风藤系列剂量组,青藤碱系列剂量组和尼莫地平组。体内实验研究对象为昆明种小鼠,共100只,雌雄各半,体质量18～24g,随机分为正常对照组,吗啡依赖模型组,青风藤组,青藤碱组和丁丙诺啡组。干预:体外实验,在吗啡依赖豚鼠回肠段的恒温浴槽中,预先加入青风藤醇提液(1,2,4g/L),青藤碱(10,50,150μmol/L)或尼莫地平(0.3μmol/L),1min后再用纳洛酮催促。体内实验,对吗啡依赖小鼠分别给予青风藤醇提液(20g/kg,灌胃),青藤碱(60mg/kg,腹腔注射),丁丙诺啡(0.4mg/kg,腹腔注射),或同体积生理盐水(腹腔注射),连续给药3d,药后30min给予纳洛酮催促。豚鼠离体回肠实验采用PCLAB生物信号采集系统测定豚鼠回肠收缩张力变化。吗啡依赖小鼠催促戒断试验观察纳洛酮催促后30min内各组小鼠跳跃反应的动物数及小鼠体质量的改变,连续观察4d。主要观察指标:①豚鼠回肠收缩张力。②小鼠跳跃反应的动物数。③大鼠体质量的变化
BACKGROUND: Qingfengteng is effective in the treatment of opioid withdrawal syndrome. However, the role and mechanism of this drug have not yet been clarified. OBJECTIVE: To investigate the effect of Sinomenine sinensis and its effective component sinomenine on the morphine-dependent withdrawal model in morphine-dependent animal models. Design: Completely randomized controlled experimental study. Unit: Department of Traditional Chinese Medicine, First Military Medical University of PLA. Subjects: In vitro studies were performed on isolated ileum of guinea pigs and divided into normal ileum control group, morphine dependent ileum group, Qingfengteng series dose group, sinomenine series dose group and nimodipine group. The in vivo experimental study consisted of Kunming mice, a total of 100 males and females, weighing 18-24 g, and were randomly divided into normal control group, morphine dependence model group, Qingfengteng group, sinomenine group and buprenorphine group. Intervention: In vitro experiments, morphine-dependent guinea pigs in the ileal segment of the constant temperature bath, pre-introduced with green ethanol extract (1,2,4 g/L), sinomenine (10,50,150 μmol/L) or nimodipine ( 0.3 μmol/L), 1 min later urging with naloxone. In vivo experiments, morphine-dependent mice were treated with alcohol extract of Rhaponinus regium (20 g/kg, intragastrically), sinomenine (60 mg/kg, intraperitoneally), and buprenorphine (0.4 mg/kg, intraperitoneally). , Or the same volume of saline (intraperitoneal injection), continuous administration of 3d, naloxone urging 30min after drug. The isolated ileum in guinea pigs was tested using a PCLAB biosignaling system to determine the change in contractile tension in the guinea pig ileum. The morphine-dependent mice were urged to take a withdrawal test to observe the change in the number of animals that responded to naloxone 30 minutes after urging in each group of mice and the changes in body weight of mice, and they were continuously observed for 4 days. MAIN OUTCOME MEASURES: 1 Ileus contraction tension in guinea pigs. 2 The number of animals that responded to the jump. 3 Changes in body weight of rats