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以嗜酸氧化亚铁硫杆菌(Acidithiobacillus ferrooxidans)为实验菌株,探讨不同条件下碳纳米管(CNTs)对其生长的影响,并采用SEM、EDS和FT-IR等手段分析CNTs对嗜酸氧化亚铁硫杆菌的毒性机制。实验结果表明,CNTs对Acidithiobacillus ferrooxidans生长有抑制作用,并随着CNTs剂量的增加,毒性增大。在CNTs投加量为500 mg/L时,培养40 h后菌株的生长量OD420达到最大值0.117,低于空白组的0.163。培养温度和培养基的pH对CNTs的细胞毒性效应有较大影响,在菌体生长的适宜条件下(pH 3.0,温度为30℃),CNTs对菌体的毒性最强。SEM、EDS和FT-IR分析结果显示,CNTs附着在细胞表面,与细胞表面的羟基、氨基等基团相互作用,并可能诱发菌体细胞产生活性氧自由基(ROS),从而导致细胞死亡。
Using Acidithiobacillus ferrooxidans as the experimental strain, the effects of CNTs on the growth of CNTs were studied. The effects of CNTs on acidophilus were analyzed by SEM, EDS and FT-IR. Toxicity mechanism of Thiobacillus ferrooxidans. The experimental results show that CNTs can inhibit the growth of Acidithiobacillus ferrooxidans, and increase with the increase of the dosage of CNTs. When the dosage of CNTs was 500 mg / L, the growth OD420 reached the maximum of 0.117 after cultured for 40 h, which was lower than that of the blank group (0.163). The culture temperature and the pH of the culture medium had a significant effect on the cytotoxicity of CNTs. Under the optimal conditions of cell growth (pH 3.0, temperature 30 ℃), CNTs had the strongest cytotoxicity. SEM, EDS and FT-IR analysis showed that CNTs attached to the cell surface and interacted with the hydroxyl, amino and other groups on the cell surface, and could induce reactive oxygen species (ROS) in cells of the cells, leading to cell death.