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目的:筛选出由黄芩、秦皮、白头翁和苦参组成的中药抗菌复方Ⅰ,并确定甲氧苄啶对其体外抗菌增效的最优添加剂量。方法:按照正交试验L9(34)设计9个组方,采用二倍稀释法测定各组方对金黄色葡萄球菌、大肠杆菌和沙门氏菌的体外抗菌效果;采用平板计数的方法,测定中药抗菌复方Ⅰ与甲氧苄啶联用作用于3种细菌1、2、4和8h后的细菌数,并计算杀菌率,并用最小二乘法对杀菌率进行拟合,计算甲氧苄啶的最优添加剂量。结果:筛选出黄芩、秦皮、白头翁和苦参按照1:4:1:2的比例组成中药抗菌复方Ⅰ,甲氧苄啶对其体外抗菌增效的最优添加剂量是2mg/g。结论:甲氧苄啶对由黄芩、秦皮、白头翁和苦参4味中药组成中药抗菌复方Ⅰ的抗菌增效作用显著。
OBJECTIVE: To screen out the antibacterial compound Ⅰ of Radix Scutellariae, Radix et Rhizoma Qinnapus, Pulsatilla Chinensis and Sophora flavescens, and to determine the optimal dosage of trimethoprim for its antimicrobial efficacy in vitro. Methods: Nine groups were designed according to the orthogonal test L9 (34). The antibacterial activity of each group against Staphylococcus aureus, Escherichia coli and Salmonella was determined by double dilution method. The antibacterial effect of traditional Chinese medicine Ⅰ combined with trimethoprim for the number of bacteria after 1, 2, 4 and 8 h of 3 kinds of bacteria, and calculate the sterilization rate, and use the least square method to fit the sterilization rate to calculate the optimal additive of trimethoprim the amount. Results: Scutellaria baicalensis, Radix Pipistrae, Pulsatilla and Sophora flavescens were screened according to the ratio of 1: 4: 1: 2. The optimal dose of trimethoprim was 2mg / g. Conclusion: The antibacterial effect of trimethoprim on traditional Chinese medicine antibacterial compound Ⅰ with Scutellaria baicalensis, Qinpi, Pulsatilla and Sophorae Flavescentis is significant.