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利用玉米基因组数据库,通过生物信息学手段鉴定玉米BURP家族基因的全序列、定位和编码蛋白,通过序列比对进行进化和分类分析,利用玉米高通量芯片表达数据进行组织表达谱分析。结果表明,玉米基因组中含有10个BURP家族基因,分布于玉米的5条染色体上,该10个基因通过选择性剪切编码14个BURP蛋白。启动子分析表明,大部分BURP家族基因的启动子区均含有逆境应答及花粉和种子发育顺式作用元件。各个发育阶段中,多数成员在生殖器官及营养器官中均有较高的表达量,只有ZmBURP3仅在营养器官根、茎、叶中高表达,ZmBURP2仅在生殖器官雄蕊和花粉中高量表达。实时荧光定量PCR结果表明,ZmBURP3、ZmBURP4基因受PEG和NaCl胁迫处理诱导不同程度上调表达。
The whole genome of maize BURP family was identified by bioinformatics analysis using the genome database of corn. The protein was located and encoded by sequence analysis, and the expression and classification of maize BURP gene was analyzed by sequence analysis. The results showed that there are 10 BURP family genes in maize genome distributed on 5 chromosomes of maize and these 10 genes encoded 14 BURP proteins by selective cleavage. Promoter analysis showed that most of the promoter regions of BURP family genes contained stress response and cis-acting elements of pollen and seed development. In all developmental stages, most of the members expressed higher levels in reproductive organs and vegetative organs. Only ZmBURP3 was highly expressed in root, stem and leaf of vegetative organs. ZmBURP2 was highly expressed in both stamens and pollen of reproductive organs. Real-time PCR results showed that ZmBURP3 and ZmBURP4 genes were up-regulated by PEG and NaCl stress.