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目的探讨RNA结合基序蛋白38(RBM38)在结直肠癌组织中的表达及其意义。方法应用免疫组化法检测90例结直肠癌及23例癌旁正常组织中RBM38蛋白的表达水平,并结合临床病理资料进行统计学分析。实时FQ-PCR法检测54例结直肠癌及对应癌旁正常组织中RBM38mRNA的表达水平。结果结直肠癌中RBM38蛋白阳性表达率低于癌旁正常组织(48.9%vs.91.3%)(P<0.01);RBM38蛋白表达水平与分化程度有关(P<0.05),而与肿瘤大小、肿瘤分期及有无淋巴结转移无关(P>0.05)。RBM38mRNA在结直肠癌组织中的表达水平低于癌旁正常组织(P<0.05)。结论 RBM38在结直肠癌组织中低表达,可能在结直肠癌发生、发展中起重要作用。
Objective To investigate the expression of RNA binding motif protein 38 (RBM38) in colorectal cancer and its significance. Methods Immunohistochemistry was used to detect the expression of RBM38 protein in 90 cases of colorectal cancer and 23 cases of adjacent normal tissues. Statistical analysis was made based on the clinicopathological data. The real-time FQ-PCR method was used to detect the expression of RBM38 mRNA in 54 colorectal cancer tissues and corresponding normal tissues. Results The positive expression rate of RBM38 protein in colorectal cancer was lower than that in adjacent normal tissues (48.9% vs.91.3%, P <0.01). The expression of RBM38 protein was correlated with the degree of differentiation (P <0.05) Staging and lymph node metastasis had no correlation (P> 0.05). The expression of RBM38 mRNA in colorectal cancer tissues was lower than that in adjacent normal tissues (P <0.05). Conclusion The low expression of RBM38 in colorectal cancer tissues may play an important role in the occurrence and development of colorectal cancer.