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目的:探讨肠源性内毒素血症(IETM)、高同型半胱氨酸血症以及氧化应激在复合致病因素法诱导的大鼠肝硬化形成过程中的作用。方法:将61只大鼠随机分为正常对照组、肝硬化模型2周组、4周组、6周组及8周组。摘取肝脏称重,计算肝系数;腹主动脉取血,测定血浆内毒素、同型半胱氨酸(HCY)、丙氨酸氨基转移酶(ALT)和总胆红素(TBil)水平;测定肝组织匀浆中肿瘤坏死因子(TNF-α),Ⅲ型前胶原(PⅢP)和总抗氧化能力(T-AOC)水平。结果:模型各组血浆内毒素、ALT活力和TBiL水平均高于对照组(P<0.05);同型半胱氨酸(HCY)随病程进展逐渐升高(P<0.05)。模型各组肝组织匀浆TNF-α、PⅢP水平明显升高,T-AOC明显降低(P<0.05)。结论:肠源性内毒素血症(IETM)、高同型半胱氨酸血症、氧化/抗氧化失平衡是肝硬化发生的重要机制。
Objective: To investigate the role of intestinal endotoxemia (IETM), hyperhomocysteinemia and oxidative stress in the formation of cirrhotic rat induced by compound pathogenic factor. Methods: 61 rats were randomly divided into normal control group, cirrhosis model 2 weeks group, 4 weeks group, 6 weeks group and 8 weeks group. The liver was weighed and the liver coefficient was calculated. Blood was taken from the abdominal aorta and the levels of plasma endotoxin, homocysteine (HCY), alanine aminotransferase (ALT) and total bilirubin (TBil) Tumor necrosis factor (TNF-α), type Ⅲ procollagen (PⅢP) and total antioxidant capacity (T-AOC) in liver homogenate were measured. Results: The levels of plasma endotoxin, ALT activity and TBiL in the model groups were all higher than those in the control group (P <0.05). The homocysteine (HCY) gradually increased with the progression of the disease (P <0.05). The levels of TNF-α and PⅢP in liver homogenate of model group were significantly increased, T-AOC was significantly decreased (P <0.05). Conclusion: Intestinal endotoxemia (IETM), hyperhomocysteinemia, and oxidative / antioxidant imbalance are important mechanisms of cirrhosis.