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AIM:To study the effect of nicotine on the migration and invasion of human esophageal squamous carcinoma cells and to investigate whether nimesulide can inhibit the effect of nicotine.METHODS:The esophageal squamous carcinoma cell line(TE-13) was treated with different concentrations of nicotine(100 μg/mL and 200 μg/mL) or 200 μg/mL nicotine plus 100 μmol/L nimesulide.Cell migration and invasion were measured using migration and invasion chamber systems.COX-2 expression was determined by Western blotting.Matrix metalloproteinase-2(MMP-2) was analyzed by zymography and ELISA.RESULTS:Nicotine(100 μg/mL, 200 μg/mL) enhanced TE-13 cells migration and invasion, and increased the protein expression of COX-2 and the activity of MMP-2.Nicotine(200 μg/mL) stimulated TE-13 cells migration and invasion which were partly blocked by nimesulide.This was associated with decreased protein expression of COX-2 and decreased activity and protein expression of MMP-2.CONCLUSION:Nicotine enhances the migration and invasion of the esophageal squamous carcinoma cell line, and nimesulide partly blocks the effect ofnicotine-enhanced esophageal squamous carcinoma cell migration and invasion.
AIM:To study the effect of nicotine on the migration and invasion of human esophageal squamous carcinoma cells and to investigate whether nimesulide can inhibit the effect of nicotine.METHODS:The esophageal squamous carcinoma cell line(TE-13) was treated with different concentrations of Nicotine (100 μg/mL and 200 μg/mL) or 200 μg/mL nicotine plus 100 μmol/L nimesulide.Cell migration and invasion were measured using migration and invasion chamber systems.COX-2 expression was determined by Western blotting.Matrix metalloproteinase -2(MMP-2) was analyzed by zymography and ELISA.RESULTS: Nicotine (100 μg/mL, 200 μg/mL) enhanced TE-13 cells migrated and invasion, and increased the protein expression of COX-2 and the activity of MMP-2. Nicotine (200 μg/mL) stimulated TE-13 cells migration and invasion which were partially blocked by nimesulide.This was associated with decreased protein expression of COX-2 and decreased activity and protein expression of MMP-2.CONCLUSION: Nicotine enhances the Migration and invasion of the esophageal squamous carcinoma cell line, and nimesulide partly blocks the effect ofnicotine-enhanced esophageal squamous carcinoma cell migration and invasion.