采用摇瓶试验,研究了淡紫拟青霉几丁质酶的形成条件。在马铃薯煎汁、玉米粉、黄豆粉3种天然基质中,以马铃薯煎汁的产酶效果最好。蔗糖、果糖、山梨糖和可溶性淀粉等碳源的添加能增加酶的产量。在氮源方面,酵母膏、蛋白胨、尿素、精氨酸等能促进酶的形成。几丁质酶可为外源几丁质所诱导,当培养液中含有0.2%的粉状几丁质时,酶的产量显著上升。淡紫拟青霉形成几丁质酶的适宜培养基初始pH为6.0～6.5,培养时间以6～8d为佳。 Shaking flask test was used to study the formation conditions of chitinase of Paecilomyces lilacinus. Among the three kinds of natural matrices, such as potato dextrin, corn flour and soybean flour, the potato juice has the best enzymatic activity. Addition of carbon sources such as sucrose, fructose, sorbose and soluble starch can increase the yield of the enzyme. In terms of nitrogen sources, yeast extract, peptone, urea, arginine and the like can promote the formation of enzymes. Chitinase is induced by exogenous chitin, and when the culture medium contains 0.2% powdered chitin, the yield of enzyme is significantly increased. Paecilomyces lilacinus chitinase formation of the appropriate medium initial pH 6.0 ~ 6.5, culture time to 6 ~ 8d is better.