论文部分内容阅读
目的:研究人参皂苷Rb1对大鼠成骨细胞增殖、分化及护骨素/核因子κB受体活化因子配体(OPG/RANKL)mRNA表达的影响。方法:不同浓度的人参皂苷Rb1作用于体外培养的大鼠成骨细胞不同时间,采用MTT法检测药物对成骨细胞增殖功能的影响,PNPP法检测成骨细胞中ALP活性分析药物对成骨细胞分化的影响,半定量RT-PCR法检测成骨细胞中OPG/RANKL mRNA,分析药物对破骨细胞分化的影响。结果:人参皂苷Rb1(1.12×10-9-1.12×10-5mol/L)作用于体外培养的大鼠成骨细胞3d时,1.12×10-8-1.12×10-6mol/L人参皂苷Rb1可显著促进成骨细胞的增殖(P<0.05或P<0.01);1.12×10-7-1.12×10-6mol/L人参皂苷Rb1可显著促进成骨细胞的分化(P<0.01);1.12×10-9-1.12×10-8mol/L人参皂苷Rb1可显著上调成骨细胞中OPG/RANKLmRNA的比值,抑制破骨细胞的分化(P<0.01);1.12×10-7-1.12×10-5mol/L人参皂苷Rb1可显著下调成骨细胞中OPG/RANKLmRNA的比值,促进破骨细胞的分化(P<0.01)。结论:人参皂苷Rb1对体外培养的大鼠成骨细胞的增殖、分化及破骨细胞的分化有显著影响。
Objective: To study the effects of ginsenoside Rb1 on the proliferation and differentiation of rat osteoblasts and the expression of OPG / RANKL mRNA. Methods: Different concentrations of ginsenoside Rb1 were used to effect osteoblasts cultured in vitro for different time. MTT assay was used to detect the effect of drugs on the proliferation of osteoblasts. PNPP assay was used to detect the effect of ALP activity in osteoblasts on osteoblasts The OPG / RANKL mRNA in osteoblasts was detected by semi-quantitative RT-PCR and the effect of drugs on osteoclast differentiation was analyzed. Results: Ginsenoside Rb1 (1.12 × 10-9-1.12 × 10-5mol / L) acts on rat osteoblasts cultured in vitro for 3d, 1.12 × 10-8-1.12 × 10-6mol / L ginsenoside Rb1 can be (P <0.05 or P <0.01); 1.12 × 10-7-1.12 × 10-6mol / L ginsenoside Rb1 could significantly promote the differentiation of osteoblasts (P <0.01); 1.12 × 10 -9-1.12 × 10-8mol / L ginsenoside Rb1 could up-regulate the ratio of OPG / RANKL mRNA in osteoblasts and inhibit osteoclast differentiation (P <0.01); 1.12 × 10-7-1.12 × 10-5mol / L ginsenoside Rb1 significantly down-regulated the ratio of OPG / RANKL mRNA in osteoblasts and promoted the differentiation of osteoclasts (P <0.01). Conclusion: Ginsenoside Rb1 has a significant effect on the proliferation and differentiation of osteoblasts cultured in vitro and the differentiation of osteoclasts.