论文部分内容阅读
目的:探讨骨髓间充质干细胞静脉移植对兔急性心肌梗死(AMI)梗死区及梗死周围区血管新生的影响及中药丹参注射液对其的干预作用。方法:用开胸结扎冠状动脉左室支的方法建立急性心肌梗死模型,将已建立心肌梗死模型的日本大耳白兔随机分为3组,模型对照组、静脉移植骨髓间充质干细胞组、静脉移植骨髓间充值干细胞联合丹参组。术后5周取材,观察梗死区内CD31(血小板/内皮细胞黏附分子)和VEGF(血管内皮生长因子)蛋白的表达。结果:1.免疫组化CD31表达,静脉注射干细胞组和干细胞联合静脉注射丹参组高于模型组(P<0.05);2.免疫组化VEGF蛋白表达,各组间无统计学差异(P>0.05)。3.血清中VEGF蛋白含量,各组间无统计学差异(P>0.05)。结论:静脉注射骨髓间充质干细胞可诱导梗死区血管新生,其机制并非通过VEGF的表达来调节的,可能是通过其他途径调节的,中药丹参注射液对干细胞促进血管新生无明显促进作用。
Objective: To investigate the effect of bone marrow mesenchymal stem cells transplantation on the angiogenesis in the infarction area and infarct area of acute myocardial infarction (AMI) in rabbits and the effect of traditional Chinese medicine Danshen injection on it. Methods: Acute myocardial infarction (AMI) model was established by ligation of left ventricular branches of the coronary arteries in thoracotomy. The Japanese white rabbits with established myocardial infarction model were randomly divided into three groups: model control group, vein grafting BMSCs group, Vein transplantation of bone marrow-derived stem cells combined with Salvia group. Five weeks after the operation, the expression of CD31 (platelet / endothelial cell adhesion molecule) and VEGF (vascular endothelial growth factor) protein in the infarct area were observed. Immunohistochemical staining of CD31, Stem cells and stem cells intravenously in Danshen group were higher than those in model group (P <0.05); 2. Immunohistochemical expression of VEGF protein showed no significant difference among groups (P> 0.05). Serum VEGF protein levels, no significant difference between the groups (P> 0.05). Conclusion: Intravenous injection of bone marrow mesenchymal stem cells can induce angiogenesis in infarction area. The mechanism is not regulated by the expression of VEGF, which may be regulated by other ways. Salvia miltiorrhiza injection has no obvious promoting effect on angiogenesis of stem cells.