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目的通过对瑞安市日常食品、公共场所、食物中毒样本中分离出的金黄色葡萄球菌进行肠毒素分型和耐药性检测,比较不同来源分离菌株的肠毒素分布和耐药性差异。方法采用ELISA方法测定金黄色葡萄球菌肠毒素(SEA-SEE),用K-B法测定分离菌株对抗菌药物的耐药性。结果 72株金黄色葡萄球菌的肠毒素检出41株,阳性率为56.94%。其中30株产2种及2种以上肠毒素。食物中毒样本、公共场所、日常食品分离株的肠毒素阳性率依次为94.44%、53.33%、33.33%。不同来源样本分离株产肠毒素阳性率差异有统计学意义(χ~2=15.94,P<0.05)。72株金黄色葡萄球菌除对左氧氟沙星、万古霉素不耐药外,对其他8种抗菌药物均存在不同程度耐药。结论金黄色葡萄球菌对多数抗菌药物存在不同程度的耐药,临床治疗应建立在体外药敏试验基础上,有针对性地选择抗菌药物。
Objective To detect the enterotoxigenicity and drug resistance of Staphylococcus aureus isolated from routine food, public places and food poisoning samples in Ruian City. The differences of enterotoxin distribution and drug resistance among different strains were compared. Methods Staphylococcus aureus enterotoxin (SEA-SEE) was determined by ELISA. The drug resistance of the isolated strains to antibiotics was determined by K-B method. Results Seventy Staphylococcus aureus enterotoxins were detected in 41 strains, the positive rate was 56.94%. Among them, 30 strains produce 2 kinds and 2 kinds of enterotoxins. The positive rates of enterotoxins in food poisoning samples, public places and daily food isolates were 94.44%, 53.33% and 33.33%, respectively. The positive rates of enterotoxigenic positive isolates from different samples were statistically significant (χ ~ 2 = 15.94, P <0.05). 72 strains of Staphylococcus aureus in addition to levofloxacin, vancomycin non-resistance, the other 8 kinds of antimicrobial drugs have varying degrees of resistance. Conclusion Staphylococcus aureus has different degrees of resistance to most antibacterials. The clinical treatment should be based on the drug susceptibility test in vitro and the antibacterial drugs should be selected.