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目的:探讨小鼠肾间质纤维化早期是否存在肾小管上皮细胞间充质细胞转型的形态学变化。方法:利用单侧输尿管梗阻的方法建立小鼠肾间质纤维化模型,常规组织学分析小鼠肾脏病理变化,免疫组化检测α- SMA的表达,肾基底膜六胺银染色和透射电镜观察肾小管基底膜的变化情况,明胶酶谱分析观察肾脏中MMP2和MMP9的表达情况。结果:成功地建立了小鼠肾间质纤维化模型,并被组织学分析证实。免疫组化分析显示肾脏中出现了大量表达α-SMA的细胞,肾基底膜六胺银染色和透射电镜分析显示小鼠肾间质纤维化早期肾脏出现了肾小管基底膜局部断裂和肾小管上皮细胞迁出的改变,明胶酶谱分析显示小鼠肾脏中MMP2和MMP9于肾间质纤维化早期有一过性增高变化。结论:肾小管基底膜局部断裂,肾小管上皮细胞迁出以及MMP2和MMP9的表达变化参与了小鼠肾间质纤维化早期上皮细胞间充质细胞转型的过程。
AIM: To investigate the morphological changes of renal tubular epithelial cell mesenchymal cells in the early stage of renal interstitial fibrosis in mice. Methods: The model of renal interstitial fibrosis was established by unilateral ureteral obstruction. The histopathological changes of kidney were observed by routine histology. The expression of α-SMA was detected by immunohistochemical staining. The renal basement membrane was analyzed by transmission electron microscopy Tubular basement membrane changes, gelatin zymography analysis of renal expression of MMP2 and MMP9. Results: Mouse renal interstitial fibrosis model was successfully established and confirmed by histological analysis. Immunohistochemical analysis showed that a large number of α-SMA cells were expressed in the kidneys. The renal basement membrane hexamine silver staining and transmission electron microscopy showed that renal tubule basement membrane local rupture and renal tubular epithelial cells Changes in cell migration, gelatin zymography analysis showed that the mouse kidney MMP2 and MMP9 in the early renal interstitial fibrosis had a transient increase. CONCLUSIONS: Local rupture of tubular basement membrane, migration of tubular epithelial cells, and changes of MMP2 and MMP9 expression are involved in the process of epithelial cell mesenchymal transition in early stages of renal interstitial fibrosis in mice.