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目的研究赤芍总苷(TSP)对失血性休克大鼠血流动力学的影响。方法用Wistar大鼠雌雄各半共24只,按照随机数字法分为4组:正常组、模型组、空白组和实验组,每组6只。4组大鼠麻醉后,都静脉注射肝素钠(2 mL·kg~(-1))全身抗凝,颈动脉插管放血,血压稳定30 min后,模型组、空白组和实验组放血至血压33.9 mm Hg,造成失血性休克模型。10 mL·kg~(-1)TSP用生理盐水配成0.5%的浓度后静脉给药,空白组给予等量的生理盐水。用生物信号采集分析系统观察大鼠的血压与心率,用自动血流变测试仪检测全血黏度、血浆黏度,红细胞变形指数、红细胞聚集指数、红细胞压积、全血还原黏度、全血相对黏度及血小板黏附率,观察TSP对失血性休克转归时血流动力学的调节作用。结果给与TSP后,实验组的全血黏度:200/s为(5.22±0.24)m Pa·s,30/s为(7.20±0.41)m Pa·s,5/s为(13.55±0.10)m Pa·s,1/s为(32.95±2.25)m Pa·s;血小板黏附率为(38.47±0.16)%,明显低于空白组的全血黏度:200/s(5.87±0.18)m Pa·s,30/s(8.46±0.23)m Pa·s,5/s(16.47±0.75)m Pa·s,1/s(41.77±1.61)m Pa·s和血小板黏附率为(39.08±0.24)%,差异有统计学意义(均P<0.05)。实验组的红细胞变形指数为0.79±0.01,明显高于空白组的0.71±0.02,差异有统计学意义(P<0.05),说明给药后红细胞变形能力明显增强。与休克时对比,用TSP复苏后,大鼠的收缩压为(106.00±13.24)mm Hg,舒张压为(76.99±13.41)mm Hg,平均血压为(80.33±8.82)mm Hg,差异有统计学意义(P<0.05)。结论 TSP能明显改善失血性休克时的血流动力学异常。
Objective To study the effects of total paeony glycoside (TSP) on hemodynamics in hemorrhagic shock rats. Methods Twenty-four male and female Wistar rats were randomly divided into 4 groups according to random number method: normal group, model group, blank group and experimental group, 6 in each group. After anesthesia, the rats in both groups were given intravenous heparin sodium (2 mL · kg -1) for systemic anticoagulation and carotid artery cannulation to exsanguinate blood. After the blood pressure was stabilized for 30 min, the model group, the blank group and the experimental group were given blood pressure to blood pressure 33.9 mm Hg, resulting in a hemorrhagic shock model. TSP 10 mL · kg -1 was dosed intravenously with 0.5% saline, while the blank group was given the same amount of saline. Blood pressure and heart rate were measured by biological signal acquisition and analysis system. Whole blood viscosity, plasma viscosity, erythrocyte deformability index, erythrocyte aggregation index, hematocrit, whole blood reduced viscosity, whole blood relative viscosity And platelet adhesion rate were observed TSP hemorrhagic shock hemodynamic outcome of the regulatory role. Results After the administration of TSP, the whole blood viscosity of the experimental group was (5.22 ± 0.24) mPa · s, (30 ± s) was (7.20 ± 0.41) m Pa · s, and that of the experimental group was (13.55 ± 0.10) mPa · s, 1 / s was (32.95 ± 2.25) mPa · s, and the rate of platelet adhesion was (38.47 ± 0.16)%, which was significantly lower than that of the blank group: 200 / s · S, 30 / s (8.46 ± 0.23) mPa · s, 5 / s (16.47 ± 0.75) mPa · s, 1 / s (41.77 ± 1.61) mPa · s and platelet adhesion rate (39.08 ± 0.24 )%, The difference was statistically significant (all P <0.05). The erythrocyte deformability index of the experimental group was 0.79 ± 0.01, which was significantly higher than that of the blank group (0.71 ± 0.02, P <0.05), indicating that the erythrocyte deformability was significantly increased after administration. Compared with the shock, the systolic blood pressure of rats was (106.00 ± 13.24) mm Hg, diastolic blood pressure was (76.99 ± 13.41) mm Hg, mean blood pressure was (80.33 ± 8.82) mm Hg after TSP resuscitation, the difference was statistically significant Significance (P <0.05). Conclusion TSP can significantly improve hemodynamic abnormality in hemorrhagic shock.