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目的 :探讨依那普利预处理对心肌内源性保护机制的影响。方法 :用依那普利预处理大鼠心肌 48h,心脏低温保存 6 h,L angendorff模型再灌注 ,观察心功能及磷酸肌酸激酶同工酶 (CK- MB)变化。结果 :依那普利预处理组左心室收缩 -舒张压差、+ dp/ dt、冠状动脉流量 (CF)等保存 6 h后功能恢复百分率明显高于、心肌 CK- MB漏出明显低于非依那普利预处理组。依那普利预处理同时加用蛋白激酶 C(PKC)抑制剂多粘菌素 B(PMB) ,保存后功能恢复降低至非依那普利预处理组水平 ;单独用 PMB预处理 ,对心功能恢复无影响。结论 :依那普利预处理大鼠心肌可以减轻心肌缺血损害 ,改善心脏保存后功能恢复。其心肌保护作用机制可能与 PKC活性有关。
Objective: To investigate the effect of enalapril preconditioning on myocardial endogenous protective mechanism. Methods: The rats were preconditioned with enalapril for 48 hours, and hearts were cryopreserved for 6 hours. Langendorff model was reperfused. The changes of cardiac function and CK-MB were observed. Results: After enalapril preconditioning, left ventricular systolic-diastolic pressure difference, + dp / dt and coronary flow rate (CF) preserved for 6 h, the percentage of functional recovery was significantly higher than that of cardiac muscle CK-MB, Pretreatment group. Pretreatment with enalapril and simultaneous administration of polymyxin B (PMB), a protein kinase C (PKC) inhibitor, restored the functional recovery to the pre-enalapril-treated group after pretreatment. Pretreatment with PMB alone Function recovery no effect. CONCLUSION: Enalapril preconditioning can relieve myocardial ischemia and improve cardiac recovery. Its myocardial protective mechanism may be related to PKC activity.