考察了外植体、培养基及光照条件对中药植物虎杖愈伤组织形成及次生代谢产物生产的影响。总的来看,所有愈伤组织中总酚和总黄酮含量比原植物的含量高2~3倍;而蒽醌的含量比原植物中含量低。外植体对愈伤组织形成及次生代谢产物生产的影响很大,所考察的3种外植体中,叶外植的愈伤组织诱导率最高而源于根外植体的愈伤组织具有最好的次生代谢能力。所考察的6种培养基中,MS+0.5mg/L2,4-D+1.0mg/L6-BA和N6+0.5mg/L2,4-D+1.0mg/L6-BA无论对于愈伤组织的产生还是次生代谢产物的累积都有较优表现。光照对愈伤组织诱导及次生代谢产物产生有明显影响,但二者无规律性联系。 The effects of explants, culture medium and light on the formation of callus and the production of secondary metabolites were studied. Overall, all the callus content of total phenols and flavonoids than the original plant content 2 to 3 times higher; anthraquinone content than the original plant content is low. The effects of explants on the formation of callus and the production of secondary metabolites were significant. Among the three explants examined, the callus induction rate was the highest in explants and the callus derived from root explants was the highest Good secondary metabolism. Among the six media examined, MS + 0.5mg / L2,4-D + 1.0mg / L6-BA and N6 + 0.5mg / L2,4-D + 1.0mg / L6- Produce or secondary metabolites have a better performance of the accumulation. Light on callus induction and secondary metabolites have a significant impact, but there is no regular correlation between the two.