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从感染马来丝虫的沙鼠腹腔收集微丝蚴(mf),以pH 7.4的PBS洗涤4次,除去腹腔细胞,低压冻干,捣碎,加PBS在4℃提取24小时,20 000转离心30分钟,测定上清液蛋白。成熟与未成熟的成虫用同法制备。解剖埃及伊蚊,收集马来丝虫感染期幼虫(L_3),超声捣碎,提取蛋白。取6～8周龄的沙鼠,每组10只,隔2周注射含10μg mf抗原的PBS 0.2ml,共2次,对照组仅注射PBS。末次注射2周后以75条L_3进行皮下
Microfilariae (mf) were collected from the abdominal cavity of gerbils infected with malayian worms and washed four times with PBS at pH 7.4 to remove peritoneal cells, lyophilized and mashed. PBS was added and extracted at 4 ° C for 24 hours and then centrifuged at 20,000 rpm Centrifuge for 30 minutes and assay the supernatant protein. Mature and immature adults prepared by the same method. The Aedes aegypti mosquitoes were dissected and larvae (L_3) were collected during the infestation of Malayi worms. The animals were sonicated and the proteins extracted. Six to eight weeks-old gerbils were housed in groups of 10 and injected with 0.2 ml of PBS containing 10 μg of mf antigen every 2 weeks for a total of 2 times. The control group received only PBS. Two weeks after the last injection of 75 L_3 subcutaneous