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目的 :研究缺氧 /复氧对小鼠大脑皮层神经细胞的损伤及参麦注射液对其保护作用。方法 :取体外原代培养 6d的小鼠大脑皮层神经细胞 ,置于 95 %N2 和 5 %CO2 的缺氧罐中造成神经细胞不同时间的缺氧复氧。用神经细胞存活率及神经细胞线粒体活性来评价神经细胞活力 ,用乳酸脱氢酶 (LDH)释放率作为评价损伤的指标 ,并进行了形态学观察。结果 :原代培养小鼠大脑皮层神经细胞缺氧 6h ,再给氧 18h后 ,神经细胞存活率及线粒体活性明显降低 ,LDH释放量显著增加。参麦注射液可显著对抗缺氧复氧致小鼠大脑皮层神经细胞的损伤 ,剂量依赖性地抑制LDH释放量的增加 ,并能减轻细胞的形态学损伤。结论 :参麦注射液对小鼠大脑皮层神经细胞缺氧复氧性损伤具有保护作用
Objective: To study the effects of hypoxia/reoxygenation on the damage of cerebral cortical neurons in mice and the protective effect of Shenmai injection. METHODS: Primary cerebral cortical neurons cultured in vitro for 6 days were exposed to hypoxia and reoxygenation at different times in 95 %N2 and 5% CO2 anoxic cans. The neuronal cell viability was evaluated using the nerve cell survival rate and the activity of nerve cell mitochondria. The release rate of lactate dehydrogenase (LDH) was used as an index to evaluate the damage, and morphological observations were performed. Results : The primary cultured mouse cerebral cortical neurons were exposed to hypoxia for 6h and then to oxygen for 18h. The survival rate of neurons and mitochondrial activity were significantly decreased, and the release of LDH was significantly increased. Shenmai injection can significantly inhibit the damage of cerebral cortical neurons induced by hypoxia/reoxygenation, inhibit the increase of LDH release dose-dependently, and reduce the morphological damage of cells. Conclusion :Shenmai Injection has a protective effect on hypoxia-reoxygenation injury of cerebral cortical neurons in mice