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本研究应用液相酶免法分析,初步揭示水稻细菌性条斑病病菌的感染途径及其在植株体内的分布规律。微量病原菌通过诸种感染途径侵染稻株后皆可在体内增殖,并传播到大部分组织器官,但数量上有差异。自然病种播种后,稻种上的病菌可传播到幼芽,根系接种后第七天,整株稻苗上可检测到增殖病菌;至孕穗期,除穗部外所有茎节和叶片均可检测到病菌,在数量上是由上至下逐渐增加。叶部接种后第三天,植株地上部有大量增殖病菌,至孕穗期部分病菌传播至穗部和茎杆中,除接种叶和其叶鞘外,植株各部位病菌分布上无明显量的差异。自然病区成熟病株上病菌主要分布在功能叶及叶鞘中,穗下茎、穗部、稻茬以及根系中亦存在少量病菌,病菌分布量由上至下逐渐减少,其余茎节中未发现病菌存在。自然病种的病菌大部份存在于种壳和种皮中,少量存在于种胚中,胚乳中无病菌存在。本研究还证明液相酶联免疫分析是非常有效而又简便的方法,但仍须改进以臻完善。
In this study, liquid phase enzyme immunoassay analysis revealed the pathogen of bacterial leaf streak (SBV) infection in rice and its distribution in plants. Micro-pathogenic bacteria through various routes of infection of rice plants can proliferate in vivo and spread to most tissues and organs, but the number of differences. After sowing of natural diseases, the germs on the rice seeds can spread to the young shoots. On the seventh day after inoculation of the root system, the multiplication germs can be detected on the whole rice seedlings. At the booting stage, all the stem nodes and leaves except the ears Detected bacteria, the number is gradually increased from top to bottom. On the third day after inoculation, a large number of pathogenic bacteria proliferated in the upper part of the plant. Some pathogens at the booting stage spread to the ear and the stem. There was no significant difference in the distribution of pathogens in all parts except the inoculated leaves and their sheaths. Pathogenic bacteria in mature wilt of natural ward were mainly distributed in functional leaves and sheaths. There were also a few pathogens in stems, ears, rice stubs and roots of the wards. The distribution of pathogen decreased gradually from top to bottom, but no other stems were found Germs exist. Most of the natural disease germs exist in the seed coat and seed coat, a small amount present in the embryo, there is no bacteria in the endosperm. This study also demonstrated that liquid phase enzyme-linked immunosorbent assay is a very effective and simple method, but it still needs to be improved to improve it.