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为探索ＨＢＶ 前Ｃ区Ａ８３ 点突变与临床关系，采用错配ＰＣＲ（ｍｐ － ＰＣＲ） 与限制性长度多态性分析（ＲＦＬＰ） 相结合的方法，对６６ 例乙肝患者的血清标本，进行了ＨＢＶ前Ｃ区Ａ８３ 点突变的检测，结果：６６ 例患者中，３６ 例（５４－５ ％ ） 检出了Ａ８３ 点突变。慢肝中度、重度、肝炎肝硬变组明显高于ＨＢＶ 携带者（ Ｐ＜ ０－０５） ，抗－ ＨＢｅ 阳性组的检出率为７３－２ ％ ，明显高于ＨＢｅＡｇ 阳性组，而这些病例中，９１－６ ％ 发生了慢性活动性肝病，提示前Ｃ区Ａ８３ 点突变的发生，可能与机体长期的炎症活动、免疫压力的选择有关。
In order to explore the clinical relationship between point A83 mutation in pre - HBV C region and HBV - DNA in 66 serum samples from hepatitis B patients by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) Pre-C region A83 point mutation detection, the results: 66 cases of patients, 36 cases (54-5%) detected the A83 point mutation. Moderate to severe chronic hepatitis, cirrhosis group was significantly higher than HBV carriers (P <0-05), anti-HBe positive group detection rate was 73-2%, significantly higher than HBeAg positive group, and these Cases, 91-6% of chronic active liver disease occurred, suggesting that the pre-C area A83 point mutation may be related to the body’s long-term inflammatory activity, the choice of immune pressure.